禽流感血凝抑制试验中鸭血清非特异性凝集因子去除方法研究  

Study on Removing the Nonspecific Agglutination Factor of Duck Serum in IH of AIV

作　　者：邓波[1,2] 李凯航[1] 薛霞[1] 孙泉云[1] 

机构地区：[1]上海市动物疫病预防控制中心,上海201103 [2]上海交通大学,上海200240

出　　处：《动物医学进展》2010年第7期39-41,共3页Progress In Veterinary Medicine

基　　金：上海市动物疫病预防控制中心科技创新资助项目

摘　　要：为解决在鸭禽流感血凝抑制试验中鸭血清与鸡红细胞的非特异性凝集,影响抗体效价的准确性,采用10 mL/L鸭红细胞直接测定法和50 mL/L鸡红细胞吸附法进行非特异性去除试验,并对结果加以比较。结果表明,10 mL/L鸭红细胞直接测定法检测血清无须前处理,具有操作简便、耗时短的优点,可用于大量样本的检测。在进行小样本、高精度要求的检测时,适宜使用50 mL/L鸡红细胞吸附+鸭细胞测定法,以更有效去除非特异性反应。The nonspecific agglutination reaction of the duck serum and the chicken red blood cell often occurred in hemagglutination inhibition to detect AIV antibody titer in duck serum,thus influencing the accuracy of antibody titer.To resolve this problem,using 10 mL/L duck RBCs directly to assay and 50 mL/L chicken RBCs to adsorb at first were both selected and compared.The first method don＇t need to fore treat serum,and have the advantages of simple protocle and short time.Using 10 mL/L duck RBCs directly to assay can be applied in large sample examinations.If we need more rigorous data,using 50 mL/L chicken RBCs to adsorb at first was more effective to remove the nonspecific reaction.

关 键 词：禽流感 非特异性凝集因子 鸭血清 

分 类 号：S855.43[农业科学—临床兽医学]