嗜群血蜱和长角血蜱ITS-2、COⅠ和COⅡ基因序列变异与亲缘关系分析  

Genetic Relationship and Sequence Variation of Haemaphysalis longicornis and H. conicinna based on ITS-2,COⅠ and COⅡ Gene Sequences

作　　者：古小彬[1] 余增莹[1] 杨光友[1] 孙家刚 魏洪 李开均 王淑贤[1] 

机构地区：[1]四川农业大学动物医学院,雅安625014 [2]四川苍溪县畜牧局,苍溪628400

出　　处：《畜牧兽医学报》2010年第6期746-754,共9页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：教育部"长江学者和创新团队发展计划"创新团队项目(IRTO848)

摘　　要：本研究旨在阐明长角血蜱与嗜群血蜱间的亲缘关系。采用聚合酶链式反应(PCR)技术从长角血蜱和嗜群血蜱基因组DNA中扩增得到核糖体第二内部转录间隔区基因(ITS-2)片段、线粒体细胞色素氧化酶亚基Ⅰ基因(COⅠ)片段和线粒体细胞色素氧化酶亚基II基因(COⅡ)片段,并进行测序,进而分析其基因变异与系统发育。结果表明,长角血蜱和嗜群血蜱的ITS-2、COⅠ和COⅡ基因片段的大小存在差异,长角血蜱3种基因分别为361、479和647bp,嗜群血蜱基因分别为354、474和661bp。长角血蜱与嗜群血蜱间ITS-2、COⅠ和COⅡ基因的相似性分别为84.2%、89.0%和88.4%。以3种基因构建的NJ进化树中,长角血蜱和嗜群血蜱均聚类。因此,认为长角血蜱和嗜群血蜱间ITS-2、COI和COII基因间变异较小,它们是血蜱属中亲缘关系较近的2个有效种,支持传统形态学的分类地位。This experiment was conducted to clarify the genetic relationship between Haemaphysalis longicornis and H. conicinna. The second internal transcribed spacer （ITS-2） of the rRNA gene, cytochrome c oxidase subunit Ⅰ （COⅠ） and cytochrome c oxidase subunit Ⅱ （COⅡ） of the mitochondrial gene were sequenced. Then, the nucleotide variability of the genes between the two tick species was compared and phylogenetic relationship was analyzed. The lengths of ITS-2, COⅠ and COⅡ in H. longicornis were 361, 479 and 647 bp, and those in H. conicinna were 354, 474 and 661 bp, respectively. The gene homology of ITS-2, COⅠ and COⅡ between H. longicornis and H. conicinna were 84.2%, 89.0% and 88.4%, respectively. Phylogenetic trees reconstructed using the NJ method based on genes ITS-2, COⅠ and COⅡ indicate that H. longicornis and H. conicinna are clustered in the same clade. The results suggest that the length of ITS-2, COⅠ and COⅡ of H. longicornis and H. conicinna are different, and H. longicornis and H. conicinna should be two different valid species with close relationship in genetics, which supports the morphologic taxonomic status of H. longicornis and H. conicinna.

关 键 词：长角血蜱 嗜群血蜱 ITS-2 COⅠ COⅡ 序列变异 亲缘关系 

分 类 号：S852.746[农业科学—基础兽医学]