西藏小型猪细胞色素b的分子遗传学研究  

The Molecular Genetic Study of Cytochrome b (Cyt b) Gene of Tibet Mini-pigs

作　　者：李洪涛[1] 肖东[2] 顾为望[2] 吴清洪[2] 袁进[2] 王万山[2] 张嘉宁[2] 

机构地区：[1]广州医学院第一附属医院,广州510120 [2]南方医科大学实验动物中心,广州510515

出　　处：《畜牧兽医学报》2010年第6期769-773,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：广东省科研计划项目(2006B60101059)

摘　　要：本研究旨在对西藏小型猪Cytb基因序列和氨基酸序列进行分析,研究其遗传分化及其与欧洲品种猪和中国几个地方猪种的亲缘关系。结果表明,西藏小型猪Cytb基因序列有9个变异位点,核苷酸多样性为0.0789。中国猪种包括西藏小型猪的Cytb基因序列与欧洲品种猪相比有16个主要突变位点,其中西藏小型猪有2个特殊碱基突变位点:420位点的T→C转换和883位点的G→A转换,几乎各占50%。氨基酸序列分析表明,西藏小型猪与欧洲品种猪有3个氨基酸位点存在着差异,其中在295位点,一部分西藏小型猪与以上其它几个品种猪同为缬氨酸(V),而另一部分西藏小型猪为异亮氨酸(I)。结果显示只有部分西藏小型猪与巴马小型猪、贵州香猪、五指山猪有很近的亲缘关系,同时证实西藏小型猪群体内存在遗传分化。To analyze the genetic differentiation of Tibet mini-pigs and its relationships with other pig breeds including European and domestic pigs, the sequences of nucleotides and amino acids of Cyt b were sequenced. The results showed that there were 9 mutation sites in Cyt b gene of Tibet mini-pigs and the nucleotide diversity was 0.078 9. Compared with pig breeds from Europe, there were 16 mutations in domestic pigs in China. There was significant difference between T-C switch at site 420 and G-A switch at site 883. The amino acid sequence analysis indicated that Tibet mini-pigs, compared with pig breeds from Europe, were different at three amino acid sites, especially at site 295, the partial Tibet mini-pigs together with other pig breeds were Valine, while another pig populations were Isoleucine at this site. The results indicated that the partial Tibet mini-pigs had very closely blood relationships with Bama Miniature pigs, Guizhou Xiang pigs and WZS pigs, and there was genetic differentiation in Tibet mini-pig group.

关 键 词：西藏小型猪 细胞色素B 基因序列 氨基酸序列 比较研究 

分 类 号：R828[医药卫生—临床医学] Q343.35[生物学—遗传学]