禽网状内皮增生病病毒对不同日龄SPF鸡的致病性比较  

Comparision on Pathogenicity of Reticuloendotheliosis Virus to Different Ages′ SPF Chickens

作　　者：孙淑红[1] 赵鹏[1] 刘绍琼[1] 崔治中[1] 

机构地区：[1]山东农业大学动物科技学院,泰安271018

出　　处：《畜牧兽医学报》2010年第6期774-777,共4页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：农业部十一五支撑计划(2006BAD06A08)

摘　　要：本研究拟通过禽网状内皮增生病病毒(REV)分子克隆化病毒REV-C99株对1、8日龄SPF鸡的致病性比较,探讨REV的致病性与感染日龄的关系。在1、8日龄SPF鸡,分别腹腔注射REV-C99株1000个TCID50.只-1,以新城疫病毒(NDV)灭活疫苗免疫后HI抗体滴度的测定结果为指标,比较了REV对不同日龄SPF鸡的致病性。结果表明,与对照组相比,1日龄SPF鸡感染REV后,即使经过NDV灭活疫苗的二次、三次免疫,对NDV的HI抗体滴度仍然差异显著(P<0.05);而8日龄SPF鸡感染REV后则随着感染时间的延长以及NDV灭活疫苗的二次、三次免疫,对NDV的HI抗体滴度差异不显著(P>0.05)。本研究表明,REV感染1日龄SPF鸡可显著抑制对NDV灭活疫苗免疫后的体液免疫反应,并且这种免疫抑制作用可持续至4个月;而8日龄SPF鸡感染REV后更多表现为一过性的致病作用。显然,REV的致病性对雏鸡感染日龄具有很强的依赖性,从而揭示出控制REV早期感染的重要性并具有现实意义。This experiment was conducted to investigate the pathogenicity of reticuloendotheliosis virus （REV） on 1 and 8-day-old SPF chickens. SPF chickens were inoculated with 1 000 TCID50·bird -1 of REV-C99, a cloned strain. The pathogenicity of REV was evaluated by the measurement of growing rate of chicks and the antibody titers to the Newcastle disease virus（NDV）. The results showed that REV challenge at a dose of 1 000 TCID50·bird-1 resulted in significantly （P0.05） suppressed HI titers of NDV untill 13-week after vaccination for 3 times. For the chicks challenge with REV at 8-d of age, however, there was no significant difference in NDV titers. The results indicated that REV challenge at 1-d of age would retard the growth of chicks and suppress the humoral immunity. The result suggests that harmful effect of REV infection is age-related and the control of REV at early age is important.

关 键 词：禽网状内皮组织增生病病毒 HI 感染日龄 致病性 

分 类 号：S852.659.3[农业科学—基础兽医学]