梗阻性黄疸对大鼠肠黏膜上皮结构及内毒素移位的实验研究  

Effect of obstructive jaundice on intestinal epithelial structure and endotoxin translation in rats

作　　者：吴鹏[1] 陈强谱[1] 张兴元[1] 

机构地区：[1]滨州医学院附属医院肝胆外科,滨州市256603

出　　处：《滨州医学院学报》2010年第3期170-172,共3页Journal of Binzhou Medical University

摘　　要：目的研究梗阻性黄疸对大鼠肠黏膜上皮结构及内毒素移位的影响。方法 30只健康雄性Wistar大鼠随机分为3组:正常对照组、假手术组、梗阻性黄疸组(OJ组)。OJ组制成梗阻性黄疸动物模型,7 d后各组同一时间点取材。观察肠黏膜上皮组织形态变化,检测门静脉血内毒素、D-乳酸含量。结果 OJ组大鼠肠黏膜上皮结构受损,门静脉血内毒素、D-乳酸水平显著高于正常对照组和假手术组(P<0.05)。结论梗阻性黄疸可导致肠黏膜上皮结构完整性受损,是发生细菌及内毒素移位的形态学基础。Objective To study the effect obstructive jaundice on intestinal epithelial structure and endotoxin translation in rats.Methods 30 male Wistar rats were assigned randomly to three groups： group control、sham and OJ.In group OJ,the common bile duct was ligated to establish the animal model.7 days later the samples of all rats were collected,the following items were detected： the level of portal vein blood endotoxin,D-lactate,pathologic morphology change of intestinal mucosa.Results The damage of the intestinal mucosa in group OJ rats was more seriously than group control and sham,and the level of portal vein blood endotoxin,D-lacate was significantly higher（all P〈.05）.Conclusion OJ could promote the damage of intestinal epithelium in rats,increase the intestinal mucosal permeability and the translocation of gut-derived endotoxin.

关 键 词：梗阻性黄疸 肠黏膜上皮 内毒素移位 

分 类 号：R392.1[医药卫生—免疫学]