贝伐单抗对兔角膜新生血管作用的影响  

Effect of Bevacizumab on corneal neovascularization in the rat model

作　　者：赵燕[1] 张少斌 张浩润 

机构地区：[1]滨州医学院眼科学教研室,滨州市256603 [2]潍坊眼科医院

出　　处：《滨州医学院学报》2010年第3期173-177,共5页Journal of Binzhou Medical University

摘　　要：目的研究贝伐单抗(Bevacizumab)在治疗兔角膜新生血管中的作用及药物浓度与作用的相关性。方法通过碱烧伤建立角膜新生血管模型,将48只健康大耳白兔随机分成A、B两组:A组为生理盐水对照组,B组为不同浓度贝伐单抗处理组(1.25、3.75、6.25、12.5、25.0 mg/ml),在裂隙灯下动态观察兔角膜新生血管生长情况,免疫组化定性观察血管内皮生长因子(vasocular endothelial growth factor,VEGF)的表达,RT-PCR基因水平下观察VEGF mRNA的表达。结果贝伐单抗对兔角膜新生血管(CNV)有抑制作用,CNV面积6.25、1.25、25.0 mg/ml贝伐单抗组与对照组、1.25、3.75 mg/ml贝伐单抗组相比,差异均有统计学意义(P<0.01),但此三组之间相比差异无统计学意义(P>0.05)。免疫组化和Real-time PCR结果显示,1.25、3.75 mg/ml贝伐单抗组与对照组相比,差异无统计学意义(P>0.05);6.25 mg/ml及以上浓度贝伐单抗组与对照组、1.25、3.75 mg/ml贝伐单抗组相比,差异均有统计学意义(P<0.01),但6.25 mg/ml以上浓度贝伐单抗组之间相比差异无统计学意义(P>0.05)。结论贝伐单抗(Bevacizumab)对兔角膜新生血管有抑制作用,且随着药物浓度增加抑制作用逐渐增强,达到一定浓度后作用趋于稳定。Objective To investigate the effects of Bevacizumab on corneal neovascularization in the rat model and its correlation between the drugs concentration and effects.Methods Corneal neovascularization was induced by 1 mol/L NaOH in rabbits（select right eye as experimental eye）.The rabbit were divided into two groups： different concentrations of Bevacizumab（1.25,3.75,6.25,12.5,25.0mg/ml）（n=8） was treated with bevacizumab drops and the other（n=8） treated with saline as control.The eyes in both groups were examined by slitlamp microscopy for new vessels at 7day.The area of CNV was marked by photography.Immunohistochemistry was used to determine the vascular endothelial growth factor（VEGF） level qualitatively of cornea with SABC method.The expression of VEGF was examined by immunohistochemistry and RT-PCR.Results The area of CNV in control group was larger than those of bevacizumab group of 6.25 mg/ml group and above（P〈0.01）.But there were no significant differences between 6.25,12.5 and 25.0 mg/ml（P〈0.05）.Immunohistochemistry and real-time PCR showed that the expression of VEGF markedly increased in control group and 1.25,3.75 mg/ml bevacizumab group while significantly suppressed in 6.25 mg/ml group and above（P〈0.01）.But there were no signifecant differences between 6.25,12.5 and 25.0 mg/ml（P〉0.05）.Conclusion Bevacizumab can inhibit corneal neovascularization significantly and the inhibition increased gradually with the drug concentration increase.The inhibition will be stable after the concentration reaches a certain role.

关 键 词：贝伐单抗 角膜新生血管 血管内皮生长因子 碱烧伤 

分 类 号：R988.1[医药卫生—药品]