银杏内酯B对动脉粥样硬化家兔模型血清CRP和主动脉VCAM-1 mRNA的影响  

Effects of ginkgolide B on serum CRP and aortic VCAM-1 mRNA in rabbit with atherosclerosis

作　　者：马轶文[1] 马东明[1] 卢斌[1] 姜希娟[1] 

机构地区：[1]天津中医药大学,300193

出　　处：《天津中医药大学学报》2010年第2期84-86,共3页Journal of Tianjin University of Traditional Chinese Medicine

基　　金：天津市自然科学基金(08JCYBJC10800);天津市卫生局基金(09KZ115)

摘　　要：[目的]探讨银杏内酯B(EGB)对动脉粥样硬化家兔模型血清反应蛋白(CRP)含量和主动脉血管细胞黏附分子-1(VCAM-1mRNA)表达的影响。[方法]通过高脂饲料喂养8周复制动脉粥样硬化家兔模型,将35只家兔随机分为对照组、模型组和银杏内酯B组,8周后,银杏内酯B组加喂EGB 5 mg(/kg.d),继续喂养8周。处死前耳缘静脉取血,采用ELISA法检测血清CRP含量;处死后取主动脉,采用实时定量PCR法检测VCAM-1 mR-NA表达。[结果]模型组血清CRP含量比对照组升高,银杏内酯B组血清CRP含量降低,与模型组相比两组差异均有显著性,P<0.01。模型组VCAM-1 mRNA表达比对照组升高,银杏内酯B组VCAM-1 mRNA表达降低,与模型组相比两组差异均有显著性,P<0.01。[结论]银杏内酯B能够降低动脉粥样硬化家兔模型血清CRP含量,有效抑制动脉粥样硬化家兔模型主动脉VCAM-1 mRNA表达,从而起到防治动脉粥样硬化的目的。[Objective] To explore the effect of ginkgolides B on the content of serum CRP and the expression of aortic VCAM 1-gene in rabbits with atherosclerosis.[Methods] High fat feed was use to induce rabbit model of atherosclerosis.Thirty-five rabbits were divided into control（ordinary）,model group,and ginkgolides B group,the last two groups were given high fat feed for eight weeks.After that the rabbits in ginkgolides B group were fed with EGB,5 mg/（kg.d）.They were continued to feed for another eight weeks.Blood sample was extracted from ear margin vein before death.Enzyme-linked immunosorbent assay（ELISA） was used to detect the rabbit serum CRP levels.After death,a sample of aortic artery was taken to detect VACM-1 gene expression by PCR method.[Results] The CRP levels of model group was obviously higher than that of control group;serum CRP content of ginkgolides B group was lower than that of model group.There was a significant difference between both groups（P0.01）.VCAM-1 gene expression in model group was higher than control,ginkgolides B group was lower than model group,obviously difference was found between two groups（P0.01）.[Conclusion] Ginkgolides B can reduce serum CRP levels in rabbits with atherosclerosis,inhibit the aortic VCAM-1 gene expression effectively,thus Preventing the development of arteriosclerosis.

关 键 词：动脉粥样硬化 银杏内酯B C反应蛋白 血管细胞黏附分子-1 

分 类 号：R285.5[医药卫生—中药学]