TGF-β1、Smad4在慢性非细菌性前列腺炎大鼠模型前列腺组织中的表达及意义  

Expressions of transforming growth factor-beta(1) and Smad4 in rat models of chronic nonbacterial prostatitis and their clinical significance

作　　者：王养民[1] 鲁守琳[1] 赵志强[1] 赵玉海[1] 王楠[1] 景德善[1] 董永超[1] 

机构地区：[1]兰州军区总医院泌尿外科,甘肃兰州730050

出　　处：《中华男科学杂志》2010年第6期490-494,共5页National Journal of Andrology

基　　金：全军科学技术项目研究"十一五"攻关课题(06G031)~~

摘　　要：目的:研究慢性非细菌性前列腺炎(CNP)SD大鼠前列腺组织中转化生长因子(TGF-β1)和Smad4的表达,并初步探讨前列腺组织纤维化的发病机制及意义。方法:将60只6月龄SD大鼠随机分为对照组(假手术)、30dCNP模型组和45dCNP模型组,每组20只。CNP模型组采用去势+高剂量苯甲酸雌二醇肌注方法建立CNP大鼠模型。用免疫组化和免疫印迹方法检查TGF-β1、Smad4在前列腺组织中的表达变化。结果:两个CNP模型组TGF-β1表达显著高于对照组(P均<0.05),而且45dCNP模型组高于30dCNP模型组(P<0.05)。两个模型组Smad4表达显著低于对照组(P均<0.05),而且45dCNP模型组低于30dCNP模型组(P<0.05)。TGF-β1、Smad4在CNP大鼠模型前列腺组织中表达呈负相关。结论:TGF-β1、Smad4可能参与CNP发病过程,前列腺纤维化可能是导致临床难以治愈的重要因素。Objective ： To investigate the expressions of transforming growth factor-beta（ 1 ） and Smad4 in the prostatic tissue of rat models of chronic nonbacterial prostatitis （CNP） , and to explore the mechanisms of CNP and its fibrosis. Methods： Sixty 6- month-old SD rats were randomly allocated into three groups of equal number： normal control, 30 d CNP model and 45 d CNP model, the models made by castration ＋ high-dose intramuscular injection of estradiol benzoate. The expressions of TGF-β1 and Smad4 in the prostatic tissue were detected by immunohistochemistry and Western blot. Results： Compared with the normal controls, the 30 d and 45 d CNP rat models showed a significantly increased expression of TGF-β1 and decreased expression of Smad4 （ P 〈 0.05 ）, even more significantly in the 45 d than in the 30 d group. And the expression of TGF-β1 was negatively correlated with that of Smad4 in the CNP rat models. Conclusion： TGF-β1 and Smad4 may be involved in the pathogencsis of CNP, and prostatic fibrosis may make the condition difficult to cure.

关 键 词：转化生长因子Β1 SMAD4 慢性非细菌性前列腺炎 纤维化 

分 类 号：R697.32[医药卫生—泌尿科学]