AMPK经过mTOR途径对大鼠血管平滑肌细胞增殖的影响  

Effect of AMPK in rat vascular smooth muscle cell proliferation through the mTOR pathway

作　　者：张萌[1] 吴峻[1] 王玮[1] 童珊珊[1] 苏楠[1] 罗福全[1] 鲁明军[1] 

机构地区：[1]广州医学院第一附属医院心血管内科,广州510120

出　　处：《山东大学学报（医学版）》2010年第6期17-21,共5页Journal of Shandong University:Health Sciences

摘　　要：目的通过研究单磷酸腺苷激活的蛋白激酶(AMPK)对血管平滑肌细胞哺乳动物雷帕霉素靶蛋白(mTOR)表达及活性的影响,探讨AMPK影响血管平滑肌细胞(VSMC)增殖的作用途径,为防治血管平滑肌细胞的异常增殖提供新的理论依据。方法组织块贴壁法培养大鼠胸主动脉VSMC,经不同浓度(0.1、0.25、0.5、1.0mmol/L)5-氨基咪唑-4-甲酰胺核糖核苷酸(AICAR)干预后,用MTT法检测VSMC增殖的变化;用Westernblot检测VSMC的p-AMPK的表达;用RT-PCR检测VSMC的mTOR的mRNA的表达,并用Western blot检测p-mTOR表达的改变。结果①与对照组相比,0.1～1mmol/L的AICAR均呈时间剂量依赖性的抑制VSMC的增殖(P<0.05);②0.5mmol/L的AICAR可以引起胞内活性p-AMPK的表达;③与对照组相比,AICAR干预组mTOR的mRNA表达及p-mTOR的活性明显受到抑制(P<0.05)。结论 AICAR可以激活VSMC中AMPK,激活的AMPK可能通过mTOR的途径抑制VSMC的增殖。Objective To investigate whether the mTOR pathway participates in the effect of AMPK on the proliferation of the vascular smooth muscle cells（VSMCs）. Methods VSMCs were primarily cultured by the explant method from the thoracic aorta of male SD rats. AICAR in different concentrations was added. Cell viability was measured by MTT assay,mTOR＇s mRNA expression by semi-quantitative RT-PCR,and p-AMPK and p-mTOR activation by Western blot. Results Compared with the control group,the AICAR intervention group could inhibit,the growth of VSMCs （P0.05）,both time-and dose-dependently; AICAR （0.5mmol/L） could activate AMPK to p-AMPK; mRNA expressions of mTOR and p-mTOR were significantly suppressed in AICAR intervention groups（P0.05）. Conclusions AICAR in different concentrations can activate AMPK by phosphorylation in VSMCs,and activation of AMPK may inhibit the VSMCs,proliferation through the mTOR pathway.

关 键 词：单磷酸腺苷激活的蛋白激酶 平滑肌细胞 血管 哺乳动物雷帕霉素靶蛋白 

分 类 号：R541.4[医药卫生—心血管疾病]