慢性心力衰竭患者外周血中Th17及Treg细胞的检测及意义  

Detection and significance of Th17 and Treg cells in patients with chronic heart failure

作　　者：李娜[1] 边红军[1] 张娟[1] 季晓平[1] 

机构地区：[1]教育部和卫生部心血管重构与功能研究重点实验室,山东大学齐鲁医院心内科,济南250012

出　　处：《山东大学学报（医学版）》2010年第6期100-103,共4页Journal of Shandong University:Health Sciences

摘　　要：目的探讨慢性心力衰竭(CHF)患者外周血中Th17细胞、CD4+CD25+Foxp3+调节性T细胞(Treg)的变化及意义。方法 49例CHF患者按照NYHA心功能分级分为CHF1组(NYHA心功能Ⅰ～Ⅱ级,n=21)和CHF2组(NYHA心功能Ⅲ～Ⅳ级,n=28)。18例健康体检者作为对照组。采用流式细胞分析法检测外周血中Th17、Treg细胞占CD4+T细胞的比例。结果 CHF1组和CHF2组患者外周血中Th17/CD4+T细胞的比例明显高于对照组(P均<0.01),CHF2组亦明显高于CHF1组(P<0.01);CHF1组和CHF2组患者外周血中Treg/CD4+T细胞的比例明显低于对照组(P均<0.01),CHF2组亦明显低于CHF1组(P<0.05);Th17/Treg的比值随心功能的减低而增高(P<0.01)。结论 CHF患者外周血中存在Th17/Treg失衡,且与心功能有一定关系,Th17/Treg失衡可能参与了CHF的发生发展。Objective To investigate the change of Th17 cells and CD4＋CD25＋Foxp3＋ regulatory T cells （Treg） in patients with chronic heart failure （CHF）. Methods The frequencies of Th17 cells and Treg cells were detected in 49 patients with CHF and 18 healthy controls by flow cytometry. The 49 patients were divided into 2 groups according to NYHA heart function class：the CHF1 group （NYHAⅠ-Ⅱ,n=21） and the CHF2 group （NYHAⅢ-Ⅳ,n=28）. Results Frequencies of Th17 cells were significantly higher in patients with poor heart function （CHF1 group and CHF2 group） than in those with normal heart function （the healthy controls） （P0.01）,and frequencies in the CHF2 group were significantly higher than in the CHF1 group （P0.01）. But change of Treg cells was totally opposite. The frequencies of Treg cells were significantly lower in the CHF1 group and the CHF2 group than in the healthy controls （P0.01）,the frequencies in the CHF2 group were significantly lower than in the CHF1 group （P0.05）,and it was confirmed that the ratios of Th17/Treg increased with decrease of heart function （P0.01）. Conclusion Th17/Treg imbalance exists in patients with CHF,so it may play a potential role in the onset and development of CHF.

关 键 词：心力衰竭 TH17细胞 CD4+CD25+Foxp3+T细胞 

分 类 号：R541.6[医药卫生—心血管疾病]