SD大鼠心肌细胞分离方法探索  

Studies on the isolation method from the cardiac myocyte of SD rats

作　　者：张紫冠[1] 李卫华[1] 黄峥嵘[1] 谢强[1] 王鑫[2] 王腾[2] 黄从新[2] 

机构地区：[1]福建医科大学附属厦门第一医院心内科,福建厦门市361003 [2]武汉大学人民医院心内科

出　　处：《心血管康复医学杂志》2010年第3期274-275,278,共3页Chinese Journal of Cardiovascular Rehabilitation Medicine

基　　金：福建省自然科学杰出青年基金(项目号2009D015);福建省教育厅科技项目(项目号JB07104);厦门市科技局科研基金(项目号3502Z20094006;3502Z20089007);厦门市卫生局科研基金(项目号WSK0613)

摘　　要：目的:探索一种稳定、简单、成功率高的,分离成年SD大鼠心肌细胞的方法。方法:采用Langendorff灌流法及二步酶解法获取单个心肌细胞,并与四步法进行比较。结果:二步法可获得横纹清晰、立体感强的单个心肌细胞,初步存活率(79.1±2.9)%,复钙后存活率(46.8±2.8)%,分别显著高于四步法的[(75.2±4.2)%,(40.2±2.2)%,P均<0.05]。与四步法相比,二步法酶解所需时间和平均试验成本明显减少[(40.2±3.3)min:(30.1±3.4)min,(115.2±5.7)元:(100.6±7.6)元,P均<0.05]。结论:该法快捷、简单、经济、重复性好,为心肌细胞的电生理研究打下良好基础。Objective.. To favor the study of electrophysiological research, a simple and reliable isolation method from cardiac myocyte of SD adult rats was investigated. Methods：The two-step enzymatic dissociation method was used to isolate myocytes from atrial and ventricular tissue on the Langendorff apparatus, and compared with four-step method for that. Results： The morphous and construction of myocardial cells was fine, the initial survival rate and survival rate after reealcification of myocardial cells respectively was （79.1 ± 2.9）%, （46.8 ± 2.8）%, in two-step method group, its were respectively more than those of four-step method [（75.2±4.2） %, （40. 2±2.2） %], P〈0. 05 all. The enzymolysis time and test cost of two-step method group were significantly less than those of four-step method group [（30. 1± 3.4） rain vs. （40.2±3.3） rain, （100.6±7.6） yuan vs. （115.2±5.7） yuan,P〈0.05 all]. Conclusion： The two-step method of cardiac myoeyte isolation is simple, stable and reduplicative, it is suited for electrophysiological experiments.

关 键 词：心肌 细胞分离 大鼠 Sprague-Dawley 

分 类 号：Q952[生物学—动物学]