同步反硝化短程除硫功能菌群的变化及鉴定  

Change and identification of functional bacteria in simultaneously denitrifying and short-cut sulfide removal process

作　　者：孙彦富[1] 刘晖[1] 刘洁萍[1] 周康群[1] 崔英德[1] 

机构地区：[1]仲恺农业工程学院环境科学与工程系,广东广州510225

出　　处：《化工学报》2010年第7期1852-1858,共7页CIESC Journal

基　　金：广东省自然科学基金项目( 06022869;07003251);广州市海珠区科技计划项目(2007-Z-023);广东省教育厅资助项目(LYM08067)~~

摘　　要：依据反硝化除硫理论,利用全混流反应器富集反硝化除硫菌,采用平板计数法和PCR-DGGE法对功能菌群进行跟踪和鉴定,并对分离纯化得到的单菌株SNB1进行鉴定。结果表明,反应器中细菌总量、除硫菌和反硝化菌分别增加了1.92×10、6.49×105和1.66×103倍。稳定运行时反应器内存在3种优势菌群。a菌群与Thauera sp.的同源性高达99%,为反硝化菌;其包含的SNB1生理生化指标显示为一株兼性自养反硝化除硫菌,且与Thauera selenatis(同源性高达99.0%)最相似;反硝化除硫菌的比例为49.3%,数量约为3.70×109cfu.ml-1。b菌群与Desulfovibrio sp.的同源性高于98%,为硫酸盐还原菌。c菌群和Rhodococcus sp.的同源性高于94%,为反硝化菌。当S/N在0.007～0.386间变化时,除S2-始终被100%转化为S0外,还有大于46.47%的SO42--S被转化为S0;而NO3-和COD的去除率分别为72.3%～95.8%和9.8%～43.5%。Based on the view about removal of sulfur by denitrifying,for which the bacteria was enriched in a continuous stirred reactor,and the functional bacteria was monitored and identified by plate count and PCR-DGGE methods.The single strain SNB1 obtained by isolation and purification of bacteria was also identified.In the reactor,the amount of bacteria for total,desulfurization and denitrifying increased by 1.92×10,6.49×105,1.66×103-fold.There are three dominant microorganisms in the reactor during stable operation.a,a flora and Thauera sp.homology up to 99.0%,and it is a kind of denitrifying bacteria.The physiological and biochemical characteristics of the SNB1 contained revealed that it is a kind of facultative autotrophic bacteria and has the 99.0% homology with Thauera selenatis.The ratio and amount of desulfurization bacteria are 49.3% and 3.70×109 cfu·ml-1.b,the homology of another flora with Desulfovibrio sp.is higher than 98%,it is sulphate-reducing bacteria.c,the homology of third flora with Rhodococcus sp.is higher than 94%,it is denitrifying bacteria.When S/N is in the range of 0.007—0.386,100% S2-and 46.47% SO2-4 are converted into S0.The removal efficiency of NO-3 and COD are 72.3%—95.8% and 9.8%—43.5%,respectively.

关 键 词：短程除硫 反硝化除硫菌 功能菌群变化 PCR-DGGE 

分 类 号：X703.1[环境科学与工程—环境工程]