丹参素对大鼠离体心脏缺血再灌注心肌能量代谢的影响  

Effect of danshensu on myocardial energy metabolism in isolated ischemia-reperfusion rat hearts

作　　者：张琳[1] 常勃勃[1] 曹婉雯[1] 曹媛[1] 杨文亮[1] 柳晓泉[1] 

机构地区：[1]中国药科大学药物代谢与动力学研究中心,南京210009

出　　处：《中国药科大学学报》2010年第3期278-282,共5页Journal of China Pharmaceutical University

基　　金：国家自然科学基金资助项目(No.30772609)~~

摘　　要：SD大鼠随机分为正常灌流组和缺血再灌注组,对大鼠离体心脏进行Langendorff灌流。采用高效液相色谱法测定大鼠心肌组织中辅酶A、乙酰辅酶A及高能磷酸化合物含量,心肌组织中乳酸含量和灌流液中脂肪酸的含量采用试剂盒测定,观察给予丹参素和阳性药雷诺嗪后上述各指标的变化情况。结果表明,雷诺嗪能够增加心肌ATP储备,升高腺苷酸能荷值,降低心肌组织乳酸含量及含脂肪酸组乙酰CoA/CoA比值,抑制脂肪酸的氧化从而优化心肌能量代谢。丹参素可以使大鼠离体心脏ATP含量及腺苷酸能荷值升高,乳酸含量降低,对正常灌流组心肌组织乙酰CoA/CoA比值无明显影响,但可以降低添加脂肪酸的缺血再灌注组心肌组织乙酰CoA/CoA比值,提示丹参素对病理状态下心肌脂肪酸的氧化有一定的抑制作用,从而优化病理状态下的心肌能量代谢,发挥对缺血再灌注心肌的保护作用。Sprague-Dawley rats were randomly divided into the normal group and ischemia/reperfusion（I/R）group.Isolated hearts were switched to the Langendorff mode and perfused with or without fatty acid.Coenzyme A（CoA）,acetyl CoA and adenine nucleotides（AMP,ADP and ATP）in heart tissues were analyzed by HPLC and lactate in heart tissues and fatty acid in perfusate were determined using the assay kit.The influence of danshensu and ranolazine on the above biomarkers were observed.Ranolazine significantly increased ATP and adenylate energy charge（AEC）of rat heart tissues and lowered lactate level as well as the ratio of acetyl CoA/CoA in rat heart tissues.Danshensu could also significantly elevate ATP and AEC values in heart tissues and lower lactate level in rat heart tissues.The ratio of acetyl CoA/CoA in I/R group with fatty acid were significantly decreased,which suggested that danshensu might inhibit myocardial fatty acid oxidation in ischemia/reperfusion rats in the presence of fatty acids.The results suggested that danshensu may partially improve myocardial energy metabolism of ischemia/reperfusion rats and protect against I/R injury of rat hearts.

关 键 词：丹参素 缺血再灌注 心肌能量代谢 离体心脏灌流 

分 类 号：R285[医药卫生—中药学]