大豆凝集素对不同种属动物T、B淋巴细胞增殖功能影响的比较研究  

Effect of Soybean Agglutinin(SBA) on Proliferation Function of T and B lymphocytes in Different Species of Animals

作　　者：王利民[1] 于吉[1] 杨树宝[1] 孙青松[1] 秦贵信[1] 

机构地区：[1]吉林农业大学动物科学技术学院,吉林长春130118

出　　处：《大豆科学》2010年第3期490-493,共4页Soybean Science

基　　金：国家自然基金项目资助项目(30471251)

摘　　要：用纯品大豆凝集素(SBA)分别对来自猪、狗、羊、兔和鸡等不同种属动物血液中的T、B淋巴细胞进行刺激,通过MTT(四甲基偶氮唑盐)比色法检测其增殖功能,同时用常规的有丝分裂源刀豆素(ConA)和脂多糖(LPS)分别刺激T和B淋巴细胞作为对比,从而研究SBA对不同种属动物免疫功能的影响。结果表明:SBA对狗、鸡和兔T和B淋巴细胞的刺激指数(SI)显著低于ConA和LPS对照组(P<0.05),对猪的SI虽低于对照组,但差异不显著(P>0.05),对羊的SI略高于对照组,差异也不显著(P>0.05)。SBA对不同动物之间T、B淋巴细胞SI的影响也有差异,其中都以鸡的最高、羊的最低为显著特征;SBA对同一种动物T和B淋巴细胞增殖功能的影响存在差异,SBA对兔、狗和猪T淋巴细胞的SI高于B淋巴细胞,而羊和鸡恰好相反。SBA对不同种属动物T、B淋巴细胞的增殖功能均有影响,尤其是对狗、兔和鸡的影响较大,而且除羊之外,其它种属动物T、B淋巴细胞的增殖功能都受到不同程度的抑制,揭示出不同种属动物免疫细胞的糖基化模式、细胞分化过程及信号传导机制不同。T and B lymphocytes from blood of different species of animals were stimulated by purified soybean agglutinin（SBA）,and detected through MTT method,while compared with ConA and LPS groups,so the effect of SBA on immune function of different species of animals could be compared.The results showed that the SI of T and B lymphocytes in dog,chicken and rabbit were significantly lower than corresponding ConA and LPS groups（P 0.05）.It was lower in pig and higher in sheep than in control group,but both had no significant difference（P 0.05）.The SI of T and B lymphocytes stimulated by SBA in different species of animals were different,and the dominant feature was that chicken were the highest and sheep were the lowest.In the same species of animals,the effects of proliferation function of T and B lymphocytes were different,the SI of T lymphocytes were higher than that of B lymphocytes in rabbit,dog,and pig,but it was just against in sheep and chicken.These results indicated that the effects of proliferation function of T and B lymphocytes stimulated by SBA in different species of animals exist,especially in dog,rabbit and chicken.Except sheep,the proliferation function of T and B lymphocytes from other species of animals were more or less inhibited.It showed that the immunocytes from different species of animals have different glycosylation mode,cytodifferentiation process and signal transmission mechanism.

关 键 词：大豆凝集素(SBA) T淋巴细胞 B淋巴细胞 增殖功能 

分 类 号：S565.1[农业科学—作物学]