Livin体外诱导人T细胞免疫的实验研究  

Experiments Study on the in vitro induce human T cell immunity

作　　者：程刚[1] 孔令斌[1] 王卫国[2] 商玲[2] 赵伟[2] 程相钦[3] 

机构地区：[1]济宁医学院临床学院,山东济宁272013 [2]济宁医学院附属第一人民医院,济宁272011 [3]山东省医学科学院消化病防治中心,济宁272000

出　　处：《济宁医学院学报》2010年第3期157-159,共3页Journal of Jining Medical University

摘　　要：目的为了探讨Livin基因对于膀胱癌细胞增殖及凋亡的调控作用。方法本研究利用Livin表达阳性的膀胱癌组织提取RNA,构建Livin特异性质粒,转染体外培养的树突状细胞(DCs),将Livin质粒转染的DCs体外与T细胞混合培养,对比分析实验组与对照组T细胞分泌INF-γ水平。结果实验组T细胞产生INF-γ(58±10)kIU/L比对照组(21±2)kIU/L明显增高(t=2.343,P<0.05)。结论Livin质粒转染的DCs对T细胞产生INF-γ具有明显的诱导作用,Livin基因对于膀胱癌细胞的增殖及凋亡有明显的调控作用。Objective To investigate the regulation effects of Livin gene to bladder cancer ceils proliferation and apoptosis.Methods We extracted RNA from livin express postive bladder cancer organization,Build livin specific plasmid,To transfection DCs in vitro,Mixed up DCs with T cells,comparison T cells secreted INF-γ levels between experiment groups and control groups INF-γ.Results INF-γ levels of experiment groups（58±10）kIU/L was significant higher than that control groups（21±2）kIU/L（t=2.343,P0.05）.Conclusion The data that Livin gene transfection DCs can significant induce T cells,increase INF-γ levels,this study suggests that Livin gene have regulation effects to bladder cancer ceils proliferation and apoptosis.

关 键 词：LIVIN 体外诱导 T细胞免疫 实验研究 

分 类 号：R392-3[医药卫生—免疫学]