罗丹明S与四溴合汞络离子缔合物固体基质室温燐光法测定痕量汞  

Solid Substrate Room Temperature Phosphorimetry for the Determination of Trace Mercury Based on Bromine Associate [(Rhod.S)_2]^(2+).[HgI_4]^(2-)

作　　者：郑志勇[1] 余奇飞[1] 郑振峰[1] 黄晓梅[1] 

机构地区：[1]漳州职业技术学院食品生物工程系,福建漳州363000

出　　处：《漳州师范学院学报（自然科学版）》2010年第2期92-96,共5页Journal of ZhangZhou Teachers College（Natural Science)

基　　金：漳州职业技术学院科研基金项目(ZZY0702)

摘　　要：基于硝酸(0.10mol/L)介质中,四溴合汞络阴离子([HgBr4]2–)与Rhod S+(罗丹明S阳离子)作用所形成的[(Rhod S)2]2+.[HgBr4]2–离子缔合物比Rhod S+.Br–在聚酰胺素膜(PAM)固体基质上所占有的Rhod S分子数增加和内重原子微扰效应,从而导致体系的室温燐光(RTP)信号剧烈增强的特性,由此建立了[(Rhod S)2]2+.[HgBr4]2–缔合物固体基质室温燐光法(SSRTP)测定痕量汞的新方法.该方法的线性范围为:0.10～16.0 fgspot–1(对应浓度0.60～40.0 pg mL–1,点样体积0.40μL),工作曲线的回归方程为△Ip=3.955+23.86mHg2+(fgspot–1),n=6,相关系数r=0.9997,检出限为5.0 agspot–1(对应浓度1.25×10–15 gmL–1).本方法成功用于人发、香烟和水样品中痕量Hg2+的测定.同时讨论了离子缔合物SSRTP测定痕量汞机理.A new solid substrate-room temperature phosphorimetry（SSRTP） for the determination of trace mercury has been established.It is based on the fact that in the acidic medium of HNO3,an ionic associate[（Rhod.S）2] ^2＋.[HgBr4]^2-will be formed by [HgBr4]^2-and Rhod.S（Rhod.S^＋）.The number of Rhod.S molecules in [（Rhod.S）2] ^2＋.[HgI4]^2-is more than that in Rhod.S^＋.Br^–,and the Hg^2＋ in [（Rhod.S）2] ^2＋.[HgBr4]^2-will induce heavy atom perturbation effect.Both aspects can cause the sharp increase of phosphorescent intensity on solid substrate.The linear range of this method is 0.10～16.0 fgspot^–1（corresponding concentration： 0.60～40.0 pg mL^–1,sample volume： 0.40 μl spot^–1） with a limit of detection of 5.0 agspot–1（corresponding concentration： 1.25×10^–15 gmL^–1）.The regression equation for working curve is △Ip = 3.955 ＋23.86mHg^2＋（fgspot^–1）,r=0.9997,n=6.This method has been successfully applied to the determination of trace Hg^2＋ in human hair,cigarette and water.The reaction mechanism for the formation of bromine associate is also discussed.

关 键 词：Hg2+ 罗丹明S 固体基质室温磷光法 

分 类 号：O629.8[理学—有机化学]