结核病患者外周血和胸水中TLR2配体活性的测定  

Assay of activity of TLR2 ligand in the peripheral blood and pleural effusion of TB patients

作　　者：陈涛[1] 张明霞[1] 邓群益[1] 朱秀云[1] 蔡雄茂[1] 李国保[1] 付向东[1] 陈心春[1] 

机构地区：[1]深圳市第三人民医院传染病研究所,广东深圳518020

出　　处：《中国热带医学》2010年第8期944-946,共3页China Tropical Medicine

基　　金：国家自然科学基金项目(30771900);广东省科学基金项目(7008514)

摘　　要：目的通过观察结核病患者外周血和胸水中IL-8的水平,检测TLR2配体的活性,探讨TLR2在结核病发病中的作用。方法选取肺结核(PTB)患者45例,结核性胸膜炎(TP)患者25例,健康对照(HD)38例,采用ELISA法分别检测血浆和胸水中IL-8含量,并用HEK293-hTLR2细胞株检测血浆和胸水中TLR2配体的活性。结果发现IL-8在结核病患者的胸水中水平明显升高(P<0.001),而且结核病人中的血浆和胸水TLR2配体的活性也高于健康者(P<0.05)。结论结核病患者感染部位TLR2配体活性及IL-8明显升高,对局部炎症细胞的浸润有重要意义,同时可作为结核病诊断的辅助手段。Aim To observe the levels of IL-8 in the peripheral blood and pleural fluid of TB patients,testing the activity of TLR2 ligands. Methods The levels of IL-8 in the peripheral blood and pleural fluid in TB patients were determined by using ELISA,and the activity of TLR2 ligand in plasma and pleural fluid with cell lines HEK293-hTLR2 was assayed. Results The level of IL-8 in pleural effusion was significantly higher in tuberculosis patients （P0.001）,and the activity of TLR2 ligands in TB patients was also higher than that in the plasma and pleural effusion of healthy donor （P〈0.05）. Conclusion The increasing expression of IL-8 and the activating of TLR2 ligands in tuberculosis infection site is of significance in local inflammatory cell infiltration.

关 键 词：结核 TOLL样受体2 IL-8 

分 类 号：R521.1[医药卫生—内科学]