低钾胁迫下不同烤烟品种根系生长和根毛形态的差异  

Study on root growth and root hair morphology in different flue-cured tobacco varieties under low potassium stress

作　　者：何伟[1] 杨中义[1] 张发明[1] 李永亮[1] 刘莉莉[1] 宋鹏飞 周冀衡[3] 

机构地区：[1]云南省烟草公司保山市公司 [2]红塔烟草(集团)有限责任公司 [3]湖南农业大学烟草工程技术研究中心,长沙市农大路1号410128

出　　处：《中国烟草学报》2010年第3期43-48,共6页Acta Tabacaria Sinica

基　　金：国家烟草专卖局资助项目(11020041012);云南省烟草公司项目(合同编号:04A02)

摘　　要：通过琼脂平板培养技术,研究了K326、NC82、云烟85、RG11、红花大金元等5个烤烟品种低钾胁迫下根系生长和根毛形态的差异。结果表明:低钾胁迫对烟苗地上、地下部生长发育影响显著。不同烤烟品种根系对低钾胁迫的响应能力具有显著性差异,其中苗期的地上部分和根系生物量表现为RG11受抑制程度最强,其次是红大和云85,NC82和K326最弱;根冠比下降幅度为RG11和云烟85>NC82和K326>红花大金元,以NC82根冠比最为协调;烤烟根系形态受抑制程度大致表现为:云烟85和RG11>K326和红花大金元>NC82;不同烤烟品种根系分布不同,RG11和红花大金元根系集中分布在2cm以下,K326分布在3cm以下,云烟85和NC82分布在3～5cm;根毛密度增加幅度大小依次为NC82>K326>云烟85>红花大金元>RG11。综合分析得出,NC82比其它4个烤烟品种耐低钾胁迫能力高,单株根系总长度和根毛长度可作为烤烟品种的耐低钾胁迫、养分利用效率及抗逆性等的重要指标,采用平板培养技术可较准确、快捷地研究烤烟种质资源的钾营养特性。Agar plating culture technology was used to study difference of root growth and root hair morphology in flue-cured tobacco varieties of K326,NC82,Yunyan87,RG11 and Honghuadajinyuan at seedling stage under low-potassium stress. Results showed that growth of up-ground part and under-ground part of seedling from different varieties was significantly influenced by low-potassium stress. The inhibition extent of low-potassium stress on dry weight of above ground part and root at seedling stage was in the sequence of RG11 Honghuadajinyuan and Yun85 NC82 and K326. There existed significant differences on response ability of roots to low-potassium stress among different varieties,and inhibition extent of root morphology roughly represented in the order of Yun85 and RG11K326 and Honghuadajinyuan NC89. The increase range of root hair density was NC82 K326Yunyan85 Honghuadajinyuan RG11,and the descent range of root-shoot ratio was RG11 and Yun85 NC82 and K326 Honghuadajinyuan,whereas the root-shoot ratio of NC82 was optimal. The results also indicated that root distribution of different varieties was discriminatory,and the roots of RG11 and Honghuadajinyuan were mainly distributed below 2cm,K326 was below 3 cm,Yunyan85 and NC82 was of between 3 and 5 cm. Therefore,low-potassium tolerance of NC82 was better than that of other 4 varieties,and total length of roots per plant and root hair is important indexes of potassium stress,nutrient use efficiency and stress resistance in flue-cured tobacco varieties,and plating culture technique could be made to research the potassium nutrition characters of flue-cured tobacco germplasm resources rapidly and efficiently.

关 键 词：烤烟 根系 根毛 苗期 平板培养 

分 类 号：S572.01[农业科学—烟草工业]