HIV-1慢性感染Jurkat细胞系的建立及其特性研究  

Establishment and characterization of HIV-1 chronically infected Jurkat cell line

作　　者：王媛媛[1,2] 王睿睿[1,2] 杨柳萌[1] 王云华[1,2] 郑永唐[1] 

机构地区：[1]中国科学院昆明动物研究所中国科学院和云南省动物模型与人类疾病机理重点实验室,云南昆明650223 [2]中国科学院研究生院,北京100039

出　　处：《中国艾滋病性病》2010年第3期218-221,共4页Chinese Journal of Aids & STD

基　　金：国家重点基础研究发展计划(973)(2009CB522306);国家科技重大专项"十一五"计划(2009ZX09501-029;2008ZX10001-002);中国科学院知识创新工程重要方向( KSCX1-YW-R-24;KSCX2-YW-R-185)~~

摘　　要：目的建立艾滋病病毒Ⅰ型(HIV-1)慢性感染Jurkat细胞系,研究其生物学特性。方法参照文献方法改良,建立HIV-1ⅢB慢性感染Jurkat细胞系。其细胞系特性采用以下方法检测:光学显微镜观察细胞形态;流式细胞仪检测HIV-1ⅢB感染细胞的阳性率;噻唑蓝(MTT)法检测抗病毒药物对慢性感染细胞的毒性作用;酶联免疫吸附试验(ELISA)检测药物对慢性感染细胞中病毒复制的影响;合胞体形成方法检测药物对HIV-1ⅢB慢性感染细胞与正常细胞融合的阻断作用。结果成功建立了HIV-1慢性感染Jurkat细胞系(Jurkat/HIV-1ⅢB),感染细胞阳性率>90%。检测影响病毒复制各周期的抗病毒药物对Jurkat/HIV-1ⅢB细胞的作用,发现Jurkat/HIV-1ⅢB细胞具有HIV-1慢性感染细胞的生物学特征。结论成功建立Jurkat/HIV-1ⅢB细胞系,为抗HIV-1药物的研发和病毒感染机制研究提供了新的工具。Objective To establish and characterize a HIV-1 chronically infected Jurkat cell line. Methods A human T lymphocyte cell line,Jurkat,was infected by the HIV-1ⅢB strain. Characterization of the chronically infected cell line was performed by following methods：the morphology was observed by microscope; the virus infected cells ratio was measured by fluorescence-activated cell sorting （FACS） analysis. The effects of anti-HIV-1 drugs on cell growth were measured by MTT method and those on HIV-1 replication in chronically infected Jurkat cell line were measured by ELISA; blockage of enfuvirtide on syncytium formation between C8166 and HIV-1ⅢB chronically infected Jurkat cells was tested. Results The results showed that HIV-1 chronically infected Jurkat cell line （Jurkat/HIV-1ⅢB） was established successfully. The ratio of infected cells was more than 90%. This cell line possessed similar characterizations of HIV-1 chronically infected cell lines by testing the effects of anti-HIV-1 drugs on such cell line. Conclusions HIV-1ⅢB chronically infected Jurkat cell line （Jurkat/HIV-1ⅢB） is established. This cell line will provide new platform for anti-HIV-1 drugs and mechanisms in HIV-1 infection.

关 键 词：艾滋病病毒 慢性感染 JURKAT细胞 

分 类 号：R512.91[医药卫生—内科学] Q291[医药卫生—临床医学]