谷氨酸氧化酶传感器芯片的研制  

Development of Glutamate Sensitive Microchip

作　　者：王荣[1] 黄春琴[1] 吴跃东[1] 章宗穰[1] 

机构地区：[1]上海师范大学化学系,200234

出　　处：《化学传感器》1999年第1期31-35,共5页Chemical Sensors

基　　金：上海市教委技术发展基金

摘　　要：采用以微电子加工技术制作的微盘铂电极,将谷氨酸氧化酶用明胶包埋法固定在该微盘电极上,制成了性能稳定的微型谷氨酸传感器。实验测定了该传感器的最佳工作电位为550mV(vs.SCE),最适溶液pH值为pH=7。在所确定的工作条件下该传感器的线性范围为0.2—6mmol·L^(-1),检测灵敏度为150nA/mmol·L^(-1)。该传感器的使用寿命大于1个月。A glutamate microbiosensor is reported in this paper. According to the need of the commercialization of biosensors, the microelectronic technology was used in the fabrication of this glutamate sensor. Glutin was used to immobilize the glutamic acid oxidase on a microdisk thin film electrode. The sensor shows a good stability for long-term using. The optimal pH value of the solution for the sensor is around 7. At the working potential of 0. 55V(vs. SCE) , the sensor also shows a good sensitivity of 150 nA/mmol·L-1,and a linear response within the range of 0. 2 -6mmol·L-1.

关 键 词：生物传感器 谷氨酸 微电极 传感器 芯片 氧化酶 

分 类 号：Q811.4[生物学—生物工程]