奇球菌pprI基因增强枯草芽孢杆菌细胞抗性的研究  

Study on Enhances of The Resistance in Bacillus subtilis by Deinococcus radiodurans pprI

作　　者：高加旺[1] 谢水波[1,2] 唐振平[2] 刘迎久[1] 刘金香[1] 唐东山[1] 

机构地区：[1]南华大学污染控制与资源化技术湖南省高校重点实验室,湖南衡阳421001 [2]南华大学铀矿冶生物技术国防重点学科实验室,湖南衡阳421001

出　　处：《南华大学学报（自然科学版）》2010年第1期78-82,共5页Journal of University of South China：Science and Technology

基　　金：国家自然科学基金资助项目(10775065);湖南省高校创新平台开放基金资助项目(09K076);湖南省重点学科基金资助项目(湘教通[2006]180号)

摘　　要：pprI是近来在奇球菌(Deinococcus Radiodurans)中发现的一个极其重要的DNA修复开关基因.本实验利用穿梭质粒pRADZ3将其转入枯草芽孢杆菌(B-1)中稳定表达,并与转化了空白质粒的菌株(B-2)对照,观察了改造后的两种菌株在H2O2氧化压力和紫外线辐照下的存活率.结果表明,在两种情况下B-1菌株存活率明显高于B-2菌株.证明奇球菌pprI基因在枯草芽孢杆菌中的稳定表达能够增强细胞抗氧化与抗紫外辐射能力.pprI,a newly identified gene switch responsible for extreme radioresistance of Deinococcus Radiodurans,plays a central regulatory role in multiple DNA damage repair and protection pathways in response to radiation stress.To evaluate whether pprI also functions in the radioresistance in other organisms,we expressed pprI in the Bacillus subtilis（B-1）using the complementation plasmid,pRADZ3-pprI,based on plasmid pRADZ3,and empty plasmid pRADZ3 was transformed to Bacillus subtilis（B-2）compared with Bacillus subtilis（B-1）.The viabilities under H2O2 oxidative stress and ultraviolet irradiation were observed.The results showed that the Bacillus subtilis（B-1）can enhance the viabilities,compared with the Bacillus subtilis（B-2）under the above two cases.It is concluded that the expression of Deinococcus Radiodurans pprI can enhance the antioxidation and antiultraviolet irradiation in Bacillus subtilis.

关 键 词：奇球菌 枯草芽孢杆菌 PPRI H2O2 紫外线 

分 类 号：Q939.11[生物学—微生物学] Q937