新疆海岛棉新海15体细胞胚胎的发生及植株再生  

Somatic Embryogenesis and Plant Regeneration in Gossypium barbadense L.XH-15

作　　者：薛金教[1] 顾冉冉[1] 袁英歌[1] 游义霞[1] 彭明[1] 崔百明[1] 

机构地区：[1]石河子大学农业生物技术重点实验室/石河子大学生命科学学院,石河子832003

出　　处：《石河子大学学报（自然科学版）》2010年第3期265-269,共5页Journal of Shihezi University(Natural Science)

基　　金：国家转基因重大专项项目(2008ZX08005-002);国家自然科学基金项目(307600991)

摘　　要：为了扩展可再生棉花的基因型,选取新疆自育海岛棉品种新海15的胚珠为外植体,利用组织培养的方法进行再生体系的建立。结果表明:含0.5mg/L KT和0.5mg/L 2,4-D的MSB培养基,在暗培养条件下可诱导胚珠,产生初始愈伤;愈伤分化则以含0.05/0.1mg/L KT和0.1mg/L 2,4-D的MSB培养基效果较佳;选取浅黄色、结构疏松的愈伤组织在含较高浓度IBA和较低浓度KT、2,4-D的3种调控培养基上依次继代,以此实现胚性愈伤组织的增殖及细胞状态调控;液体悬浮培养和干燥胁迫处理均有利于胚性愈伤的体细胞胚胎诱导;子叶胚的萌发及植株再生则以含谷氨酰胺和天冬酰胺的1/2 MSB培养基效果较佳。Ovule of Xinhai 15,a breed of Gossypium barbadense cultured in Xinjiang,were used as explant to figure out a favorable culture system for the regeneration of somatic embryo.The result indicates that Calli of ovule were effectively produced on the medium with 0.5mg/L Kinetin（KT） and 0.5mgL 2,4-Dichlorophenoxyacetic acid（2,4-D） in darkness.The best culture media for callus differentiation is MSB media supplemented with 0.05/0.1mg/L KT and 0.1mg/L 2,4-D.To produce embryogenic calli and control the cell statue,light yellow,loose callus were cultured on three different control medias supplemented with high concentration of IBA and low concentration of KT and 2,4-D one after another.Liquid suspend and dehydration are beneficial to the emergence of somatic embryo.Plantlets were effectively regenerated on the 1/2 MSB media supplemented with L-Glutamine and L-Asparagine.

关 键 词：海岛棉新海15 胚珠 愈伤组织 胚性愈伤组织 体细胞胚胎 植株再生 

分 类 号：Q751[生物学—分子生物学] S562.035[农业科学—作物学]