植物染色体C-带带型制作技术的改良  

Improving the Methods for Metaphase Chromosome C-bands in Plants

作　　者：郭红梅[1] 朱伟伟[1] 陈福龙[1] 陈芳[1] 陈远良[2] 高剑峰[1] 

机构地区：[1]石河子大学生命科学学院,石河子832003 [2]石河子蔬菜研究所,石河子832000

出　　处：《石河子大学学报（自然科学版）》2010年第3期270-273,共4页Journal of Shihezi University(Natural Science)

基　　金：国家人事部出国留学人员重点科技项目

摘　　要：利用黄瓜新泰密刺种子根尖为材料,制作黄瓜中期染色体C显带制片,得到清晰稳定的染色体C显带带纹,在采用常规压片和去壁低渗的制片方法的基础上,对植物染色体显带的制片方法进行了改进。结果显示,利用改良后的方法得到了较多的且清晰稳定的黄瓜中期染色体C显带带纹,其单倍染色体带纹总数为30。结论:染色体制片中的解离、压片、干燥等多个步骤对染色体显带有显著的影响。To obtain the chromosome with the clear and stable C-bands,the root tip of xintaimici cucumber was used.Based on conventional sheeting method,disassociating cell wall hypoosmotic method,improving the method for observation of chromosome in plant.The result is that the more clear and stable chromosome C-bands imagine were obtaind by using the improved method.The single time the number of the band on single chromosome is 30.The conclusion is that the steps of dissociation,sheeting and drying have marked effect on chromosome C-bands.

关 键 词：染色体 C带 改良 

分 类 号：Q343[生物学—遗传学]