IGF-1在慢性间歇性缺氧大鼠脑缺血再灌注表达的实验研究  

Empirical study on expression of IGF-1 in rats with chronic intermittent hypoxia combining cerebral ischemia reperfusion

作　　者：李植灿[1] 洪志林[1] 蔡若蔚[1] 

机构地区：[1]福建医科大学附属第二医院,泉州362000

出　　处：《福建医药杂志》2010年第3期60-62,F0003,共4页Fujian Medical Journal

摘　　要：目的探讨慢性间歇性缺氧(CIH)对大鼠脑缺血再灌注(CIR)胰岛素样生长因子-1(IGF-1)表达的影响。方法 SD大鼠46只,随机选取10只在缺氧箱中行血氧检测,余随机分成4组:空白对照组(UC),假手术组,CIR组,CIH+CIR组。建立SD大鼠CIH模型,并行局灶性脑缺血再灌注干预,采用免疫组化法观察额顶部皮质缺血半暗带IGF-1的表达,并进行2,3,5-三苯基氯化四氮唑染色观察脑梗死体积。结果 CIH+CIR组大鼠神经功能缺损及梗死体积较CIR组严重(P<0.05);CIH+CIR组与CIR组相比较IGF-1表达减少(P<0.05)。结论 IGF-1是脑缺血再灌注损伤的保护性因子,CIH可使IGF-1表达降低,并可能是CIH加重脑缺血再灌注损伤的机制之一。Objective To explore the effect of chronic intermittent hypoxia（CIH）on insulin-like growth factor-1（IGF-1）in rats with cerebral ischemia-reperfusion（CIR）injury.Methods Forty-six Sprague-Dawley（SD）rats were chosen.Ten rats were carried out randomly and the arterial blood oxygen saturation of them was detected in hypoxic box.The remained thirty-six rats were randomly assigned to four experimental groups：unhandled control group（UC）,sham operation group,CIR group,CIH combining CIR group.The CIH model was established,and then the rats were exposed to the focal middle cerebral artery occlusion.The expression of IGF-1 were detected by immunohistochemistry.Infarct size was also investigated by TTC staining.Results The neurological functional deficit and the infarct volume in CIH combining CIR group were more than those in CIR group（P0.05）.The expression of IGF-1 in CIH combining CIR group was less than that of CIR group（P0.05）.Conclusion IGF-1 is protective factors in cerebral ischemia reperfusion injury.CIH decreases the expression of IGF-1,which may be one of the mechanisms of CIH enhancing cerebral ischemia reperfusion injury.

关 键 词：阻塞性睡眠呼吸暂停综合征 脑梗死 慢性间歇性缺氧 脑缺血再灌注 胰岛素样生长因子-1 

分 类 号：R766[医药卫生—耳鼻咽喉科]