稀有放线菌的分离及抗菌筛选  

Isolation and Screening of Antagonistic Rare Actinomycetes

作　　者：范丽霞[1] 郑继平[1] 

机构地区：[1]海南大学食品学院微生物实验室,海南海口570228

出　　处：《海南师范大学学报（自然科学版）》2010年第2期185-187,共3页Journal of Hainan Normal University（Natural Science）

基　　金：海南省教育厅高等学校科研项目(Hjkj2008-06)

摘　　要：通过对海南不同植被的土壤、对土样不同的预处理方式及抑制剂的有无对稀有放线菌进行分离、筛选出57株稀有放线菌,使用纸片扩散法测定它们对大肠杆菌、枯草芽胞杆菌、金黄色葡萄球菌、黑曲霉的拮抗作用.结果表明,从不同植被土壤分离的稀有放线菌数量上看,耕作土>草甸土>风沙土;土样不同的预处理后稀有放线菌的检出中,自然风干>化学处理>烘干处理;土壤共有8个菌株对指示菌有拮抗作用,其中3株具有进一步研究的价值.In the paper,we get 57 strains rare actinomycetes by isolation from the HaiNan soil of different vegetation、 different pretreatment to the soil samples and having inhibitor or not. The slip of paper diffusion is used to measure the antagonistic action of the separated rare actinomycetes to Escherichia coli、Bacillus subtilis、Stephylococcus aureus and Aspergillus niger.The result indicated that the order of the quantity of rare actinomycetes of different vegetation is cropped soil 〉 meadow soil 〉 aeolian 〉 sandy soil; the order of the quantity of rare actinomycetes of different pretreatment to the soil samples is natural air drying 〉 chemical treatment 〉 furnace drying treatment. There are eight strains rare actinomycetes which have antagonistic action to the indicator, and three of them is worthy of further study.

关 键 词：稀有放线菌 分离 拮抗作用 

分 类 号：Q939.13[生物学—微生物学]