颗粒裂解肽抗菌结构域在大肠杆菌中表达水平的分析  

Analysis of the level of expression of antimicrobial domains of granulysin in E.coli

作　　者：周鹏[1] 查向东[1] 

机构地区：[1]安徽大学生命科学学院,合肥230039

出　　处：《生物学杂志》2010年第3期48-52,共5页Journal of Biology

基　　金：安徽省高校省级自然科学研究重点项目KJ2007A091

摘　　要：为实现颗粒裂解肽抗菌结构域的高效表达并避免其对宿主菌的毒性,研究设计了共表达阴离子配体重组生产阳离子抗菌肽的方法。结果证明该方法可有效提高阳离子抗菌肽的表达产量。同时研究分析了颗粒裂解肽不同抗菌结构域在同一原核表达载体pACYCDuet-1中表达水平的实验数据。通过计算机程序对mRNA二级结构的模拟分析,计算并比较出其二级结构生成自由能之间的差异,提出了预测颗粒裂解肽抗菌结构域在E.coli中表达水平的几点依据,参考这些依据可以对不同抗菌肽基因进行改造以获得高效表达。In order to explore a new approach for high expression of the different antimicrobial domains of granulysin and to avoid the toxic effects on Escherichia coli host cells during its expression,a novel strategy for the production of recombinant cationic antimicrobial peptides was designed by co-expressing an anionic ligand.The results demonstrated that a higher expression yield was achieved by using the co-expression method.Some experimental data about the expression levels of the domains in vector pACYCDuet-1 were also analyzed.The mRNA secondary structures were predicted simultaneously by computer-aided design.Results of the predictive analysis showed the difference between the energy of mRNA secondary structures.According to some references,we suggest a set of judgements.It will be helpful for us to modify these antimicrobial domains before experiment.

关 键 词：阳离子抗菌肽 阴离子配体 共表达 二级结构 翻译起始区 

分 类 号：Q786[生物学—分子生物学]