中国大陆不同自然隔离群钉螺的同工酶谱分析  

ANALYSIS ON THE ISOENZYME PATTERNS AMONG THE NATURAL ISOLATES OF ONCOMELANIA SNAIL IN China's Mainland

作　　者：王少海[1,2] 何立[1,2] 康在彬 

机构地区：[1]湖北药检高等专科学校寄生虫学教研室 [2]湖北医科大学寄生虫学教研室

出　　处：《中国寄生虫病防治杂志》1999年第1期52-55,共4页Chinese Journal of Parasitic Disease Control

基　　金：国家自然科学基金

摘　　要：本文对中国大陆的福建、湖北、云南、四川４个自然隔离群的１３个县钉螺进行酯酶同工酶（ＥＳＴ）和苹果酸脱氢酶（ＭＤＨ）电泳酶谱比较，ＥＳＴ共出现１０～１４条酶带，ＭＤＨ共出现３～４条酶带。比较它们的酶谱特征可见：（１）同一省内不同地区间钉螺的酶谱非常相似，具有较密切的亲缘关系；（２）四川、云南２省钉螺的酶谱特征差异较小，而与福建、湖北２省的酶谱比较，差异明显；（３）４个自然隔离群钉螺呈现为３个地理分布种群，分别分布于福建、湖北和滇川３个区域，各群体共有的ＥＳＴ酶带有３条，ＭＤＨ酶带仅１条，其它酶带数目和电泳迁移率均呈现明显差异。Four natural isolates of Oncomelania snail, field collected from 13 counties of Fujian, Hubei, Yunnan and Sichuan Provinces in the mainland of China were studied in the aspects of esterase(EST) isoenzyme and malate dehydrogenase (MDH) isoenzyme by slab polyacrylamida gel electrophoresis. 10-14 enzyme bands were separated on EST. 3-4 enzyme bands were separated on MDH. The characteristic of isoenzyme patterns was comparated carefully. The results showed that: 1. The isoenzyme patterns were very resemblance in different regions of same province, it revealed that sibling relationship existed in these regions. 2. The characteristic of isoenzyme patterns was similar between smails of Yunnan and Sichuan Provinces, but it showed obvious difference with snails of Fujian and Hubei Provinces. 3. Four natural populations of snail might be classified into three geographical strains according to the isoenzyme patterns, i.e. Fujian, Hubei and Yunnan Sichuan. 3 enzyme bands on EST and one on MDH were completely identical among three geographical strains, but other enzyme bands appeared apparent difference at number of enzyme bands, enzyme activity and Rf values. These results showed that the genetic variation existed among three strains.

关 键 词：钉螺 种群 同工酶 亲缘关系 血吸虫 

分 类 号：R184.38[医药卫生—流行病学]