来源于沿叶脉褪绿症状桃的PLMVd分离物群体结构分析与序列测定  

Population structure and sequence analysis of a Peach latent mosaic viroid isolate from a peach tree showing discoloration along leaf veins

作　　者：徐文兴[1] 窦瑞木[2] 王国平[1] 洪霓[1] 王振华 

机构地区：[1]华中农业大学植物科技学院,武汉430070 [2]河南农业职业学院植物科学系,中牟451450 [3]湖北省出入境检验检疫局,武汉430022

出　　处：《植物病理学报》2010年第3期317-321,共5页Acta Phytopathologica Sinica

基　　金：国家自然科学基金项目(No.30800746)

摘　　要：Peach latent mosaic disease occurred in peach trees is distributed widely in China.The disease shows a wide range of symptoms in the fields and discoloration along leaf veins is easily found.To get insight into the correlation between the symptom and Peach latent mosaic viroid(PLMVd),leaves of a peach tree showing the symptom of discoloration along its veins were collected and subjected to PLMVd RNA extraction,and RT-PCR detection.The target amplified fragment was cloned,and some positive clones were then selected for analyzing population structure of the isolate by single-strand conformation polymorphism(SSCP) analysis.Three predominant clones of the isolate were sequenced and analyzed.This study would provide more information for understanding the correlation between the molecular characterization of PLMVd and the symptom of discoloration along leaf veins.Peach latent mosaic disease occurred in peach trees is distributed widely in China.The disease shows a wide range of symptoms in the fields and discoloration along leaf veins is easily found.To get insight into the correlation between the symptom and Peach latent mosaic viroid（PLMVd）,leaves of a peach tree showing the symptom of discoloration along its veins were collected and subjected to PLMVd RNA extraction,and RT-PCR detection.The target amplified fragment was cloned,and some positive clones were then selected for analyzing population structure of the isolate by single-strand conformation polymorphism（SSCP） analysis.Three predominant clones of the isolate were sequenced and analyzed.This study would provide more information for understanding the correlation between the molecular characterization of PLMVd and the symptom of discoloration along leaf veins.

关 键 词：桃 病虫害防治 结构分析 序列测定 

分 类 号：S662.1[农业科学—果树学] S436.3[农业科学—园艺学]