菌寄生真菌纤细齿梗孢一丝氨酸-苏氨酸蛋白激酶基因OPK1的克隆及序列分析  

Cloning and characterizing of the gene OPK1 encoding a serine-threonine protein kinase in mycoparasite fungi Olpitrichum tenellum

作　　者：赵培宝[1,2] 任爱芝[2] 李多川[1] 

机构地区：[1]山东农业大学植物保护学院,泰安271018 [2]聊城大学植物保护系,聊城252000

出　　处：《植物病理学报》2010年第3期329-332,共4页Acta Phytopathologica Sinica

基　　金：国家自然科学基金(30571246);山东省教育厅项目(J08LF56)

摘　　要：Using degenerate PCR and TAIL-PCR,a protein kinase gene OPK1(Genebank accession No.:EU417815) was cloned from mycoparasite fungi Olpitrichum tenellum.OPK1 has an open reading frame of 2 031 bp interrupted by two introns(108 bp,84 bp) and putatively encodes a protein of 676 aa.Phylogenetic analysis indicated that OPK1 was most similar to other serine-threonine protein kinase in fungi.Southern blot analysis indicated that OPK1 is present as a single copy in the genome.RT-PCR showed it could be transcribed both in the phase of spore germination and hyphal growth.Using degenerate PCR and TAIL-PCR,a protein kinase gene OPK1（Genebank accession No.：EU417815） was cloned from mycoparasite fungi Olpitrichum tenellum.OPK1 has an open reading frame of 2 031 bp interrupted by two introns（108 bp,84 bp） and putatively encodes a protein of 676 aa.Phylogenetic analysis indicated that OPK1 was most similar to other serine-threonine protein kinase in fungi.Southern blot analysis indicated that OPK1 is present as a single copy in the genome.RT-PCR showed it could be transcribed both in the phase of spore germination and hyphal growth.

关 键 词：蛋白激酶基因 菌寄生真菌 苏氨酸 丝氨酸 序列分析 有丝分裂原活化蛋白激酶 细胞内信号转导 可逆磷酸化 

分 类 号：S852.655[农业科学—基础兽医学]