甘蔗黄叶病毒复制酶基因的克隆及序列分析  

Cloning and sequencing analysis of RNA-dependent RNA polymerase of Sugarcane yellow leaf virus

作　　者：王勤南[1,2] 高三基[1] 陈如凯[1] 刘绍谋[2] 金玉峰[2] 

机构地区：[1]农业部甘蔗遗传改良重点开放实验室,福州350002 [2]广州甘蔗糖业研究所,广州510316

出　　处：《植物病理学报》2010年第3期333-336,共4页Acta Phytopathologica Sinica

基　　金：农业部"948"计划项目(2006-G37);公益性行业科研专项(3-9);现代农业产业技术体系建设专项资金

摘　　要：The gene of RNA-dependent RNA polymerase(RdRP) was cloned by RT-PCR from Sugarcane yellow leaf virus-Fuzhou isolate(CHN-FJ1) and then cloned into pMD18-T vector.The sequence showed that the fragment comprised 1 212 nucleotides including part gene of ORF1 and ORF2.The ORF2 was involved the RdRP gene consisted of 995 nucleotides and encoded putative protein of 331 amino acids.Compared the nucleo-tide sequence and encoded putative protein of CHN-FJ1 with the other isolates from different countries,they shared the homology above 92.0%.Phylogenetic tree suggested that the sixteen isolates were classified into four types according to the amino acid sequence of the RdRP.One of the groups contained CHN-FJ1 and the other isolates from China,American,Brazil,Australia and Colombia;however,there was the closest relation between CHN-FJ1 and BRA-YL1 isolate from Brazil.The gene of RNA-dependent RNA polymerase（RdRP） was cloned by RT-PCR from Sugarcane yellow leaf virus-Fuzhou isolate（CHN-FJ1） and then cloned into pMD18-T vector.The sequence showed that the fragment comprised 1 212 nucleotides including part gene of ORF1 and ORF2.The ORF2 was involved the RdRP gene consisted of 995 nucleotides and encoded putative protein of 331 amino acids.Compared the nucleo-tide sequence and encoded putative protein of CHN-FJ1 with the other isolates from different countries,they shared the homology above 92.0%.Phylogenetic tree suggested that the sixteen isolates were classified into four types according to the amino acid sequence of the RdRP.One of the groups contained CHN-FJ1 and the other isolates from China,American,Brazil,Australia and Colombia;however,there was the closest relation between CHN-FJ1 and BRA-YL1 isolate from Brazil.

关 键 词：甘蔗糖业 黄叶病毒 复制酶基因 序列分析 克隆 VIRUS 可持续发展 LEAF 

分 类 号：S435.121.9[农业科学—农业昆虫与害虫防治]