细菌脱氢酶活性生物测试法用于水质毒性检测的改进  

Improvement on the Determining Method of Dehydrogenase Activity in Water Toxicity

作　　者：陈莉[1] 梁文艳[1] 李炯[1] 郭思远[1] 

机构地区：[1]北京林业大学环境科学与工程学院,北京100083

出　　处：《中国环境监测》2010年第3期17-21,共5页Environmental Monitoring in China

基　　金：国家自然科学基金(No50678024);国家高技术研究发展计划(No2007AA06Z301)

摘　　要：从培养基配方、菌种来源、激活时间、菌液用量以及检测方式等几个方面对脱氢酶快速毒性检测法进行了改进,确定了整个体系的操作方法。并用HgCl2和NaN3验证了改进后方法的可行性。结果表明,以天然地表水作为菌种来源,转接培养1次后,混合细菌的脱氢酶活性的稳定性便可达到实验要求。样品液与菌液按照6∶1的比例混合效果较好。混合体系在37℃恒温培养箱中激活50min以上细菌可完全活化,最终检测可用普通分光光度计进行。改进后的方法解决了原方法菌液失活过快,实验稳定性较差的问题,同时克服了检测仪器难以购买的问题,使这一方法得以在普通实验室实现。The paper studied the bioassay method of dehydrogenase activity and improved the experiment conditions,such as the cultrure medium,the source and amount of bacteria,the activation time as well as detection method.The operating conditions and method were determined.The feasibility of the improved method was verified by the bioassay of HgCl2 and NaN3.The results showed that when natural surface water was used as bacteria source,the dehydrogenase activity of bacteria could keep higher after transferring culture for one time.The optimum mixing ratio of water sample to bacteria suspension liquid was 6∶1（V∶V）.Bacteria activity could be fully activated when mixed system of preserved bacteria and cultrue medium were placed in 37℃ for more than 50min.The improved method overcame the short preservatin time and stability for bacteria and could be realized in ordinary laboratory by the use of spectrophotometer.

关 键 词：脱氢酶 混合细菌 毒性检测 生物测试 

分 类 号：X832[环境科学与工程—环境工程]