我国云南省艾滋病病毒感染的长期不进展者人免疫缺陷病毒1型tat基因的序列分析  

Sequence analysis of human immunodeficiency virus type 1 tat gene among the long term HIV infected non progressoris in Yunnan Province

作　　者：赵峰 邵一鸣 段一娟[1] 陈小萍 赵全壁[1] 苏玲[1] 管永军[1] 曾毅[1] 

机构地区：[1]中国预防医学科学院病毒学研究所艾滋病参比实验室

出　　处：《中华实验和临床病毒学杂志》1999年第1期37-40,共4页Chinese Journal of Experimental and Clinical Virology

基　　金：国家科委技术规划(863)课题

摘　　要：目的人类免疫缺陷病毒１型（ＨＩＶ－１）ｔａｔ基因是该病毒的调控基因之一。本研究是探讨ｔａｔ基因变异是否影响ＨＩＶ－１感染者的病程进展。方法从云南ＨＩＶ流行区的２２例ＨＩＶ感染后临床进程不同的人抽取外周血，提取核酸，用套式聚合酶链反应（ＰＣＲ）扩增ＨＩＶ－１的ｔａｔ基因，并进行了核酸序列测定和分析。结果２２个ＨＩＶ毒株中，有１１株来自长期不进展者，均为Ｂ亚型。另外１１株来自一般感染者的ＨＩＶ毒株，５株为Ｂ亚型，６株为Ｃ亚型。长期不进展者和ＨＩＶ－１Ｂ亚型一般感染者的ｔａｔ基因序列与国际Ｂ亚型共享序列比较，两组均在相同的固定的４个位点上氨基酸与共享序列不一致。在系统树分析中两组亦聚集在一起，未显示出分离。Ｃ亚型一般感染者的ｔａｔ基因序例组内变异小，在固定的６个位点上与国际Ｃ亚型共享序列不一致。结论长期不进展者与一般Ｂ亚型感染者的Ｔａｔ蛋白序例均未发现规律性差异。ＨＩＶ感染后的进展速率可能与ＨＩＶ－１ｔａｔ基因序例无明显关系。Objective HIV 1 tat gene is one of its regulatory genes necessary for its riplication. This study is to explore whether the variation of tat gene influences the AIDS progress of HIV infectect individual. Methods We carried out investigation on tat gene of HIV among the long term HIV infected non progressors and the commonly infected individuals. Mononuclear cells of peripheral blood were isolated and cellular DNA was extracted. Nested PCR method was used to amplify tat gene and their sequences were analyzed. Results Blood were collected from 22 HIV infected individuals, of which 11 cases were infected with B subtype strains based on previous env gene analysis and belonged to long term non progressors according to clinical features and immunology. The other 11 cases were common HIV infection of which 5 subtype B and 6 subtype C were found. Both long term non progressor group (subtype B) and the commonly HIV infectedly group (subtype B) had constantly four amino acids different from that of international B subtype consensus sequence The both groups clusteed randomly on the phylogenetic tree. In commonly HIV infected subtype C group there were 6 amino acids different from that of international subtype C. The variation inside the tatgene of common subtype C group was small.Conclusions There was no regular variation and significant difference between long term HIV infected non progressve group and commonly HIV infected subtype B group. There is no evidence showing that progressive speed of AIDS correlated with the tat sequence profile of HIV 1.

关 键 词：HIV-1 TAT基因 序列分析 B亚型 C亚型 

分 类 号：R512.910.2[医药卫生—内科学] R373.9[医药卫生—临床医学]