丙型肝炎病毒(HCV)E2/NS1相对保守区多肽抗原在检测抗-HCV抗体中的意义  

Significance of polypeptide antigen of HCVE2/NS1 relative conservation region in detection of anti HCV

作　　者：李群辉[1] 胡翔鹄[1] 闵福援[1] 刘惠珍[1] 汪俊韬[1] 

机构地区：[1]北京佑安医院

出　　处：《中华实验和临床病毒学杂志》1999年第1期61-63,共3页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的研究（ＨＣＶ）Ｅ２／ＮＳ１相对保守区多肽抗原在检测抗－ＨＣＶ中的意义。方法利用ＨＣＶＥ２／ＮＳ１基因编码的膜区糖蛋白合成Ｅ２／ＮＳ１相对保守区多肽抗原，建立酶免疫试验（ＥＩＡ），对９６例ＨＣＶ感染者及４０例正常献血员进行抗ＨＣＶＥ２／ＮＳ１抗体的检测，同时平行检测ＨＣＶＲＮＡ和丙氨酸转氨酶（ＡＬＴ）。结果抗ＨＣＶＥ２／ＮＳ１在ＨＣＶ感染者的检出率为４１．６７％，其中急性丙型肝炎为１３．５５％，慢性丙型肝炎为２５．０４％，无症状感染者为２．０８％；正常献血员中发现３例抗ＨＣＶＥ２／ＮＳ１抗体弱阳性，而ＨＣＶＲＮＡ阴性；对抗ＨＣＶＥ２／ＮＳ１与ＨＣＶＲＮＡ比较，两者的共同阳性率为６２．５％，抗ＨＣＶＥ２／ＮＳ１与ＨＣＶＲＮＡ具有相关性；在抗ＨＣＶＥ２／ＮＳ１阳性的急慢性患者中ＡＬＴ值之间有明显差异（Ｐ＜０．０５），急性比慢性高；抗ＨＣＶＥ２／ＮＳ１与ＲＮＡ共同阳性率中慢性感染明显高于急性感染。结论抗ＨＣＶＥ２／ＮＳ１阳性同时也有病毒核酸存在，但抗ＨＣＶＥ２／ＮＳ１相对保守区抗体可能有一定的中和作用，这种体液免疫在清除ＨＣＶ感染中有一定的作用，抗ＨＣＶＥ２／ＮＳ１相对保守区抗体是否就是中和抗体仍有待进一步研究?Objective In order to study the significance of HCVE2/NS1 relative conservation region antigen.Methods EIA was established with HCVE2/NS1 relative conservation polypeptide antigen, as well as serum HCV RNA and ALT in patients with HCV infection. Results The positive rate of 96 cases patients who were infected by HCV is 41.67%, acute 13.55% and chronic 25.04%,and we found that there were 3 cases weak positive in 40 healths; Agreement rate is 62.5% in 40 cases of the positive of anti E2/NS1 in sera with HCVRNA, and they have relation. In ALT level in sera, acute infection of HCV is higher than chronic (P<0.05).Conclusion There are virus necleic acid in sera when anti HCVE2/NS1 apparence positive; however, antibody of anti E2/NS1 relative conservation region has the some neutralive actions, and this humor immunity may have the actions in clearing virus, but wether anti body of anti E2/NS1 relative conservation of HCV is or not neutralive antibody well be investigated further.

关 键 词：丙型肝炎 病毒抗体 RNA 中和抗体 HCV 

分 类 号：R373.21[医药卫生—病原生物学]