庚型肝炎病毒IgM抗体检测方法的建立及其临床意义  

Detection of IgM antibody to hepatitis G virus and its clinical significance

作　　者：刘洪[1] 貌盼勇[1] 洪世雯[1] 胡燕[1] 白雁平[1] 鞠连才[1] 

机构地区：[1]解放军302医院

出　　处：《中华实验和临床病毒学杂志》1999年第1期77-79,共3页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的建立一种早期、快速诊断庚型肝炎的血清学检测方法。方法以辣根过氧化物酶标记庚型肝炎病毒（ＨＧＶ）多肽ＮＳ３，ＮＳ５区段抗原，建立了捕获酶联免疫吸附试验（ＥＬＩＳＡ），用于检测血清中ＨＧＶＩｇＭ抗体。结果本法不受特异性ＩｇＧ的竞争和类风湿因子的干扰；与其它致肝炎的病毒（ＨＡＶ、ＨＢＶ、ＨＣＶ、ＨＥＶ、ＣＭＶ、ＥＢＶ）无交叉反应。检测４６例非甲、乙、丙、戊型肝炎患者血清，抗－ＨＧＶＩｇＭ阳性１４例，阳性率３０．４３％，其中，６例同时为ＨＧＶＲＮＡ阳性，阳性符合率为４２．８６％（６／１４），检测１２例庚型肝炎病人双份血清，其中，急性期血ＨＧＶＩｇＭ抗体均为阳性。结论该法检测ＨＧＶＩｇＭ抗体特异性强，敏感性高，且简便快速，适用于临床对庚型肝炎新近感染的早期诊断，有推广应用价值。Objiective To provide a rapid and early serodiagnostic technique for the patients with hepatitis G virus(HGV). Methods A capture ELISA was developed to detect anti HGV IgM in sera using synthetic peptides of HGV NS3 NS5 gene regions conjugated with horseradish peroxidase.Results This assay was not disturbed by competition of specific IgG or interfered by RF and did not cross react with HAV、HBV、HCV、HEV、CMV and EBV. Among 46 sera of non A E hepatitis,14 were positive for anti HGVIgM, the positive rate was 30.43%,6 was positive for HGV RNA the positive rate was 42.86%. 12 paired sera from acute stage of HGV patients were all positive by capture ELISA. Conclusion This method is sensitive, specific, rapid and stable for detecting anti HGV IgM, and is useful in the early diagnosis of HGV infection.

关 键 词：庚型肝炎病毒 IGM ELISA 

分 类 号：R373.21[医药卫生—病原生物学] R446.61[医药卫生—基础医学]