单链多聚核苷酸的喇曼光谱及其主要谱带指认  

On the Study of Single-strand Polynucleotides by Raman Spetroscopy

作　　者：廖昱博[1] 赖昭胜[1] 许永强[1] 

机构地区：[1]赣南师范学院物理与电子信息学院,江西赣州341000

出　　处：《赣南师范学院学报》2010年第3期38-40,共3页Journal of Gannan Teachers' College(Social Science(2))

基　　金：赣南师范学院自然科学研究项目(08kyz04)

摘　　要：研究了4种单链多聚核苷酸(PolyA,PolyU,PolyG,PolyC)的喇曼光谱,分析了不同盐溶液浓度对核酸主链构象的影响,并给出了各种单链核酸主要谱带的初步指认.研究表明,喇曼光谱对于研究核酸的氢键配位、主链构象和碱基堆积来说是一种强劲的工具.In this paper,Raman spectroscopic technique was used to study four kinds of synthesized polynucleotides,i.e.PolyA,PolyU,PolyG,PolyC.The effects of different salt concentrations in solution on the backbone conformation of the nucleic acids were analyzed,and tentative Raman assignments were also given to the main bands of these synthesized polynucleotides.The results suggest that Raman spctroscopy is a strong tool for probing hydrogen bonding,local conformation,and base stacking of nucleic acids

关 键 词：碱基 多聚核酸 拉曼光谱 

分 类 号：O657.37[理学—分析化学]