外源Ca^(2+)对南林895杨扦插苗生长及稳定同位素δ^(13)C,δ^(15)N动态分布的影响  

Effects of Exogenous Ca^(2+) on Growth and Dynamic Distribution of Stable Isotope δ^(13)C,δ^(15)N of Populus × euramericana‘Nanlin 895’Cuttings

作　　者：王雷[1] 尹增芳[1] 尤禄祥[1] 李建银[1] 于明祥[1] 徐棉芬[1] 

机构地区：[1]南京林业大学森林与环境学院,南京210037

出　　处：《林业科学》2010年第6期51-57,共7页Scientia Silvae Sinicae

基　　金：国家自然科学基金(30671657)资助项目

摘　　要：利用形态检测与稳定同位素技术,分析不同质量浓度外源Ca2+(CaCl2)处理下,南林895杨扦插苗株高、地径、生物量的变化情况以及各营养器官δ13C,δ15N值的动态变化。结果表明:不同质量浓度的外源Ca2+均能不同程度地促进苗木的生长。外源喷施Ca2+后,扦插苗株高、地径、生物量均优于对照,其中以200mg·L-1质量浓度的Ca2+处理效果最好,其单株平均株高、地径、生物量分别比对照增加14.82%,18.01%,19.7%。随着Ca2+质量浓度(0,2,20,100,200,300mg·L-1)的升高,南林895苗木叶、茎、根中δ13C,δ15N值均呈现逐渐上升然后缓慢下降的趋势,且最大值出现在Ca2+质量浓度为200mg·L-1处。δ13C,δ15N值在植株中的分布遵从"库"器官大于"源"器官的规律。相关性分析表明:"源"器官δ13C,δ15N值与株高、地径呈显著性负相关,与生物量呈正相关;"库"器官δ13C,δ15N值与株高、地径、生物量均呈显著正相关。不同营养器官δ13C,δ15N值的动态变化在一定程度上可以反映南林895杨扦插苗"源""库"器官间光合同化物积累与再分配格局对外源Ca2+质量浓度的响应。In this paper,growth in height,caliper,and biomass,and dynamic changes of the δ^13C and δ^15N value in the vegetative organs of Populus × euramericana＇Nanlin 895＇cuttings were investigated in response to different concentrations of exogenous Ca^2＋ treatments. The experimental results showed that different concentrations of exogenous Ca^2＋ were able to promote the plant growth; the height,caliper,and biomass increments were all greater than the control after spraying exogenous Ca^2＋ ,among which the 200 mg·L ^-1 Ca^2＋ concentration exhibited the best effect. Compared with the control, the average of the height,caliper and biomass treated with 200 mg·L^ -1 Ca^2＋ increased by 14. 82% ,18. 01% ,and 19. 7% respectively. The stable isotope,δ^13C and δ^15N,enrichment capacity of the Populus × euramericana＇Nanlin 895＇ cuttings were affected by different concentration exogenous Ca^2＋ to various degrees. With the Ca^2＋ concentration increased （0,2,20,100,200,300 mg·L ^-1）,the δ^13C and δ^15N values of the leaf,stem and root presented a gradual increase at low concentrations,and then slowly declined at high concentration,with the maximum value appeared at the concentration of 200 mg·L ^-1 Ca^2＋ . The distribution of δ^13 C and δ^15 N value in the plant complied with the principle that the ＂sink＂ organ was more than the＂source＂organ. The correlation analysis showed that both the δ^13C and δ^15N values of＂source＂ organ had a significantly negative correlation with the height,caliper,and the positive correlation with biomass. The δ^13C, δ^15N value of＂sink＂organ had a significantly positive correlation with the plant height,caliper,and biomass. The δ^13C, δ^15 N value dynamic changes of different vegetative organs reflected to a certain extent accumulation and redistribution patterns of the photoassimilates in the＂source＂and＂sink＂organs of Populus × euramericana ＇Nanlin 895＇cuttings in response to exogenous Ca^2＋ concentration.

关 键 词：南林895杨 外源CA^2+ 生物量 Δ^13C δ^15N 

分 类 号：S723.13[农业科学—林木遗传育种]