HPLC法测定清肝化瘀合剂中槲皮素的含量  

Determination of quercitrin in qingganhuayu mistura by HPLC

作　　者：徐月萍[1] 孙梁燕[1] 许永军[1] 

机构地区：[1]浙江省慈溪市人民医院药剂科,慈溪315300

出　　处：《海峡药学》2010年第6期50-52,共3页Strait Pharmaceutical Journal

基　　金：浙江省慈溪市科技计划项目;项目编号CR200710

摘　　要：目的建立清肝化瘀合剂中槲皮素含量测定的HPLC方法。方法采用高效液相色谱仪,使用Hypersil ODS2色谱柱(4.6×250mm,5μm),流动相为甲醇-0.4%磷酸水溶液(51∶49),流速为1.0mL.min-1,检测波长为360nm,柱温为25℃。结果槲皮素在2.9～23.22μg.mL-1呈良好线性关系(r=0.9999),槲皮素平均加样回收率为99.12%,RSD为1.81%。结论该方法简便,快捷,准确度高,可用于清肝化瘀合剂中槲皮素的含量测定。OBJECTIVE To establish a HPLC method for determine quantitatively quercitrin in qingganhuayu mistura.METHODS The column was Hypersil ODS2（4.6×250mm,5μm）;the mobile phase was methanol-0.4% phosphoric acid solution（51∶49,V/V）;the flow rate was 1.0mL·min-1;the detective wavelength was 360 nm;the column temperature was 25℃.RESULTS The linear range of quercitrin was 2.9～23.22μg·mL-1（r=0.9999）,the average recovery was 99.12%,and RSD was 1.81%;CONCLUSION The method is simple,rapid,accurate,and can be applied to the determination of quercitrin in qingganhuayu mistura.

关 键 词：清肝化瘀合剂 槲皮素 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]