固相萃取-HPLC法测定延年康口服液中二苯乙烯苷的含量  

Determination of Stilbene Glucoside in Yanniankang Koufuye by SPE-HPLC

作　　者：郭炎荣[1] 路玫[1] 

机构地区：[1]广西壮族自治区人民医院,南宁530021

出　　处：《海峡药学》2010年第6期78-80,共3页Strait Pharmaceutical Journal

摘　　要：目的建立延年康口服液中二苯乙烯苷的含量测定方法。方法采用固相萃取-HPLC法,色谱柱为XTerra RP18(5μm 3.9mm×150mm),流速0.8 mL.min-1,乙腈∶水(20∶80)为流动相,检测波长为320nm。结果二苯乙烯苷的线性范围为0.0504μg～0.3024μg,r=0.9992,平均回收率为98.77%,RSD=1.64%(n=6)。结论方法简便、快速、准确,适合该口服液中二苯乙烯苷的含量测定。OBJECTIVE To establish a method for the determination of Stilbene glucoside in Yanniankang Koufuye.METHODS SPE-HPLC was conducted on a XTerra RP18 column（5μm,3.9mm×150mm） with acetonitrile-water（20∶80）,flow rate 0.8mL·min-1,detecting at 320nm.RESULTS The linear was 0.0504μg～0.3024μg（r=0.9992）,the average recovery was 98.77%,RSD=1.64%（n=6）.CONCLUSION The method was simple,sensitive,accurate and suitable for the determination of Stilbene glucoside in Yanniankang Koufuye.

关 键 词：固相萃取-HPLC法 延年康口服液 二苯乙烯苷 

分 类 号：R927.2[医药卫生—药学]