黄芪甲甙在Balb/C小鼠CVB3病毒性心肌炎中抗凋亡作用的机制  

Anti-apoptosis Effect and Mechanism of Astragaloside on Viral Myocarditis in Balb/C Mice With CVB3

作　　者：罗永姣[1] 邓晖[1] 李双杰[1] 杜九中[1] 

机构地区：[1]南华大学第一附属医院,湖南衡阳421001

出　　处：《南华大学学报（医学版）》2010年第3期328-331,共4页Journal of Nanhua University(Medical Edition)

摘　　要：目的观察黄芪甲甙对Balb/C小鼠CVB3病毒性心肌炎心肌细胞凋亡的影响,探讨黄芪甲甙抗心肌细胞凋亡的可能机制。方法 Balb/C小鼠50只随机分5组(每组10只)。空白对照组:腹腔无菌注射不含病毒的Eagle’s培养基0.1 mL,在腹腔注射病毒培养基30 min后,以生理盐水0.1 mL灌胃,共7天;病毒性心肌炎对照组:小鼠每只腹腔注射0.1 mL内含1×105TCID50CVB3的Eagle’s培养基,在腹腔注射含病毒的Eagle’s培养基30 min后,以生理盐水0.1 mL灌胃,共7天;黄芪甲甙低、中、高剂量干预组:在腹腔注射病毒30 min后,用黄芪甲甙[具体剂量分别为0.07、0.2、0.6 mg/(kg.d)]0.1 mL灌胃,共7天。采用缺口末端标记法(TUNEL)检测心肌凋亡细胞,免疫组织化学检测Bcl-2、Bax蛋白的表达,逆转录聚合酶链反应(RT-PCR)检测Bcl-2、Bax基因的表达,并利用图像分析系统测量平均光密度值进行半定量分析。结果与空白对照组比较,病毒性心肌炎对照组心肌细胞凋亡发生率增高;抑制凋亡因子Bcl-2基因及蛋白表达下降,而促进凋亡因子Bax基因及蛋白表达增强,Bcl-2/Bax mRNA比值降低。与病毒性心肌炎对照组比较,黄芪甲甙高剂量干预组能降低CVB3病毒性心肌炎心肌细胞的凋亡指数,Bcl-2基因及蛋白水平表达增强,Bax基因及蛋白水平表达下降。结论黄芪甲甙在Balb/C小鼠CVB3病毒性心肌炎中抗凋亡作用机制可能是促进了抑制凋亡基因Bcl-2表达,抑制了促凋亡Bax基因表达。Objective To study the anti-apoptosis effect and mechanism of Astragaloside on viral myocarditis in mice with CVB3.Methods Fifty Balb/C mice were randomized into five groups（n=10）：normal control group,given 0.1 mL of EMEM by intraperitoneal injection,were treated with saline 0.1ml with gavage after 30 minutes of injection for 1 week;viral myocarditis control group,inoculated intraperitoneally with 0.1 mL of 1×102 TCID50 CVB3 diluted in Eagle＇s minimal essential medium（EMEM） solution were given saline 0.1ml with gavage after 30 minutes of injection for 1 week;astragaloside low-dose intervention group,middle-dose intervention group and high-dose intervention group,inoculated intraperitoneally with 0.1 mL of 1×102 TCID50 CVB3 diluted in Eagle＇s minimal essential medium（EMEM） were treated with 1%,3%,9% astragaloside（0.07,0.2 and 0.6 mg/（kg·d）,respectively） 0.1mL solution after 30 minutes of injection,respectively with gavage for 1 week.Apoptosis of cardiomyocyte and levels of Bax,Bcl-2 were detected respectively by insitu end-labeled DNA（TUNEL）,reverse transcription-polymerase chain reaction（RT-PCR） and immunohistochemistry method. Results The apoptosis index was significantly higher in viral myocarditis group than that in control group,the levels of Bcl-2 mRNA and protein was lower,the levels of Bax mRNAand protein was higher,the ratio of Bcl-2 /Bax mRNA was lower.High dose Astragaloside significantly reduced the apoptosis index of viral myocarditis,induced the expression levels of Bcl-2 protein and mRNA,reduced the expression level of Bax protein and mRNA than that in viral myocarditis group respectively.Conclusion Astragaloside could reduce the apoptosis of viral myocarditis,which mechanism may be partly up-regulated expression of Bcl-2 and down-regulated expression of gene Bax.

关 键 词：黄芪甲甙 抗凋亡 病毒性心肌炎 Bcl-2基因 BAX基因 

分 类 号：R542.21[医药卫生—心血管疾病]