磁性纳米粒子固定葡萄糖氧化酶修饰电极电致化学发光葡萄糖传感器  

A Novel Electrochemiluminescence Biosensor Based on Glucose Oxidase Immobized on Magnetic Nanoparticles

作　　者：熊志刚[1] 李建平[1] 唐丽[2] 陈志强[1] 

机构地区：[1]桂林理工大学化学与生物工程学院,桂林541004 [2]桂林理工大学附属医院,桂林541004

出　　处：《分析化学》2010年第6期800-804,共5页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(No.20665003);教育部科学研究重点项目(No.207087);广西自然科学基金(No.0728214)资助项目

摘　　要：通过交联剂将葡萄糖氧化酶(GOD)固定在Fe3O4磁性纳米粒子上,在磁力作用下将该磁性复合粒子修饰在石蜡碳糊电极(SPCE)表面,制成易更新酶电极。GOD催化氧化葡萄糖生成过氧化氢,并使鲁米诺产生电致化学发光(ECL),据此首次构建了易更新型电致化学发光葡萄糖传感器。其电致发光强度与葡萄糖浓度在1×10-5～1.0×10-2mol/L范围内呈线性关系,线性回归方程I=65.4374C+23.9017(r=0.9987);检出限为1.0μmol/L。此传感器响应快,稳定性高,表面易更新,已用于测定人血清中葡萄糖的含量。A novel Nano-Fe3O4 particles biosensor for glucose based on luminol electrochemiluminescence （ECL） is presented. Glucose oxidase （GOD） was covalently cross-linked to the surface of synthesized magnetic nano-Fe3O4 particles. Then the composite particles Fe3O4/GOD was adhered to solid paraffin carbon paste electrode by magnetic force to fabricate a working electrode. Hydrogen peroxide was produced by enzymatic reaction of GOD and ECL could be obtained by the reaction between luminol and hydrogen peroxide to construct the renewable glucose biosensor. There was a linear relationship between ECL and glucose concentration in the ranges of 1×10-5-1.0×10-2 mol/L with a regression equation of I=65.4374C＋23.9017（r=0.9987）, and the detection limit is 1 μmol/L. The ECL biosensor has showed short response time and high stability, and the electrode surface was renewable easily. The proposed biosensor has been applied to the determination of glucose in plasma samples.

关 键 词：电致化学发光 磁性纳米粒子 葡萄糖氧化酶 鲁米诺 化学修饰电极 

分 类 号：R446.1[医药卫生—诊断学]