银杏叶提取物对大鼠哮喘模型中Cyclin D1表达的影响  

Effects of Ginkgo biloba Extract on the expression of Cyclin D1 in asthmatic rats

作　　者：刘广河[1] 吴宏欣[2] 丁艳霞[1] 李勉[1] 

机构地区：[1]河南大学药学院,河南开封475004 [2]河南大学校医院,河南开封475001

出　　处：《中国民族民间医药》2010年第13期19-20,共2页Chinese Journal of Ethnomedicine and Ethnopharmacy

摘　　要：目的:探讨银杏叶提取物对大鼠哮喘模型中Cyclin D1表达的影响及其机制。方法:将60只大鼠随机分为正常组、哮喘组和干预组,应用卵蛋白激发大鼠建立哮喘气道重塑模型。采用免疫组化技术检测大鼠气道中Cyclin D1的表达。结果:哮喘组中可见气道壁有明显炎症细胞浸润,气道壁的厚度增加,Cyclin D1表达水平显著高于正常组和干预组(P<0.05)。干预组Cyclin D1表达水平较哮喘组明显下降。结论:Cyclin D1参与了哮喘气道重塑的发生,银杏叶提取物可能通过影响Cyclin D1的表达进而影响气道重塑过程。To explore： the effects of ginkgo biloba extract on the expression of Cyclin D1 and its mechanism in the asthmatic rats. Methods ： 60 Wistar rats were randomly divided into 3 groups ： normal group, asthmatic group and intervention group ; the model of asthmatic rats with airway remodeling was induced with OVA. Immunohistochemistry method was used to detect the expression of Cy- clin D1. Results： In the asthmatic group, the infiltration of inflammatory cells, thicknesses of airway walls and expression of Cyclin D1 were significantly increased compared with those of the other two groups （ P 〈 0. 05 ）. Conclusion ： Cyclin D1 may take part in the de- velopment of the asthmatic airway remodeling. Ginkgo biloba extract can prevent the development of airway remodeling by effecting the expression of Cyclin D1.

关 键 词：银杏叶提取物 细胞周期蛋白D1 哮喘 气道重塑 

分 类 号：R282.5[医药卫生—中药学]