高效液相色谱法测定复方洛美沙星即型凝胶滴耳剂中地塞米松含量  

Determination of Dexamethasone in Compound Lomefloxac in Situ Forming Gels Ear Drops by HPLC

作　　者：陈丽娜[1] 李东 谢燕贤[4] 温中明 廖朝峰 李惠民[1] 

机构地区：[1]广东省深圳市宝安区人民医院,广东深圳518101 [2]广东省深圳市人民医院 [3]暨南大学医学院第二临床医学院,广东深圳518020 [4]广东省深圳市宝安区石岩医院,广东深圳518020 [5]广东省深圳市光明新区公明医院,广东深圳518020

出　　处：《中国药业》2010年第12期36-37,共2页China Pharmaceuticals

基　　金：深圳市科技计划项目(医疗卫生类);项目编号:200903179

摘　　要：目的采用高效液相色谱法测定复方洛美沙星即型凝胶滴耳剂中地塞米松的含量。方法以Diamonsil C18柱为色谱柱,流动相为乙腈-水(40:60,pH为3.0～3.5),流速为1mL/min,柱温40℃,检测波长240nm,进样量20μL。以峰面积外标法计算。结果洛美沙星质量浓度在0.015～0.036mg/mL范围内与峰面积线性关系良好,r=0.9999(n=5),平均回收率为98.01%,RSD=2.95%(n=9)。结论该法操作简便、快速,结果准确可靠,重现性好,可用于测定复方洛美沙星即型凝胶滴耳剂中地塞米松的含量。Objective To establish a HPLC method for the determination of dexamethasone in Compound Lomefloxacin Ear Drops.Methods The Diamonsil C18 column was used as an analytical column.The mobile phase was acetonitrile-water(40:60) with the flow rate of 1 mL/min,the detective wavelength was 240 nm.The external standard method was used.Results The standard curve of dexamethasone was linear in the concentration range of 0.015-0.036 mg /mL(r = 0.999 9),the average recovery rate was 98.01%,RSD = 2.95%(n = 9).Conclusion The HPLC method is simple,rapid and accurate for the determination of dexamethasone in Compound Lomefloxacin Ear Drops.

关 键 词：洛美沙星 地塞米松 即型凝胶 滴耳剂 含量测定 

分 类 号：R927.2[医药卫生—药学] R987