两个波长处测定阿替洛尔有关物质的比较  

Comparison of Detecting Related Substances of Atenolol by Two Wavelengths

作　　者：刘红莉[1] 姜建国[1] 宋更申[1] 马钱钱[2] 

机构地区：[1]河北省药品检验所,河北石家庄050011 [2]山西长治医学院,山西长治046000

出　　处：《中国药业》2010年第12期50-51,共2页China Pharmaceuticals

摘　　要：目的采用两个波长分别测定阿替洛尔的有关物质,并对结果进行分析。方法采用高效液相色谱(HPLC)法,色谱柱为十八烷基硅烷键合胶柱,以磷酸盐缓冲液(取磷酸二氢钾6.8g,加水溶解并稀释至1000mL,用磷酸调pH至3.0)700mL、加甲醇300mL与辛烷磺酸钠1.30g混匀作为流动相,检测波长为226nm或275nm,流速为1.0mL/min。分别在两个波长处测定有关物质。结果在226nm波长处所测得的数据准确,误差小。结论在226nm波长处测定阿替洛尔有关物质,可更有效地控制产品的质量。Objective To determine the related substances of atenolol by two wavelengths and to analyze the results.Methods High performance liquid chromatography（HPLC） was carried out,using the column packed with octadecylsilance silica gel and the mixture of 700 mL of phosphate BS（dissolving 6.8 g of potassium dihydrogen phosphate with water and diluting to 1 000 mL,adjusting to pH 3.0 with phosphoric acid）,300 mL of methanol and 1.30 g of sodium octanesulfonate as the mobile phase.The detection wavelength was set at 226 nm and 275 nm.The flow rate was 1.0 mL/min.Results The related substances were separately determined at two wavelengths,the measured results at 226 nm wavelength was accurate with small error.Conclusion Measuring atenolol related substances at 226 nm can more effectively control the quality of the product.

关 键 词：检测波长 阿替洛尔 片剂 有关物质 

分 类 号：R927.1[医药卫生—药学] R972.4