河南省汉族人群基质金属蛋白酶-1基因-519A/G多态性及其血浆水平与急性冠状动脉综合征的相关性分析  

Study on the relationship between the serum level and gene polymorphism of matrix metalloproteinase-1 and acute coronary syndrome in the Han nationality from Henan province

作　　者：王浩坤[1] 赵洛沙[1] 周帅[1] 陈艳[1] 宗永华[1] 王杨[1] 高敏[1] 张静[1] 

机构地区：[1]郑州大学第一附属医院心内科,郑州450052

出　　处：《临床心血管病杂志》2010年第5期371-374,共4页Journal of Clinical Cardiology

摘　　要：目的:探讨河南省汉族人群中基质金属蛋白酶-1(MMP-1)基因-519A/G多态性及其血浆水平与急性冠状动脉综合征(ACS)的相关性。方法:采用多聚酶链反应-限制性片断长度多态性分析(PCR-RFLP)结合琼脂糖凝胶电泳技术检测295例ACS患者、105例稳定型心绞痛(SAP)患者和198例健康对照者MMP-1基因-519A/G多态性;采用酶联免疫吸附法(ELISA)测定血浆MMP-l水平,比较2组之间MMP-1基因多态性频率分布的差异和血浆水平的差异。结果:①ACS组MMP-1基因AA型所占比例明显高于SAP组及对照组,差异具有统计学意义(P<0.05);A等位基因频率在ACS组、SAP组和对照组分别为90.5%、83.3%、83.6%,ACS组与SAP组和对照组比较,差异亦有统计学意义(P<0.05)。②ACS组血浆MMP-l水平高于SAP组和对照组(P<0.05)。③不同基因型之间血浆MMP-1水平差异无统计学意义。结论:MMP-1基因-519A/G多态性可能与河南省汉族人群ACS发病相关;血浆MMP-l水平与冠状动脉斑块不稳定性相关。Objective：To investigate the relationship between the serum level and gene polymorphism of matrix metalloproteinase-1（MMP-1） and acute coronary syndrome（ACS） in the Han nationality from Henan province.Method：Two hundred and ninety nine patients with ACS,105 patients with SAP and 198 normal controls were enrolled and examined by the method of polymerase chain reaction-restriction fragment length poly-morphism（PCR-RFLP） for the-519 A/G polymorphism of MMP-1 gene.The serum level of MMP-1 was measured by enzyme linked immunosorbent assay（ELISA）.All of the people in the study were in the Han nationality from Henan province.Result：①The frequency of AA genotype was significantly higher in patients with ACS than that in SAP or controls（P〈0.05）.②The serum level of MMP-1 in patients with ACS was higher than that in SAP group or controls（P〈0.05）.③There were no differences in the serum level of MMP-1 between different genotypes.Conclusion：Tn the Han nationality from Henan province,the MMP-1 gene-519A/G polymorphism was associated with the risk of ACS,and the serum level of MMP-1 was related to the plaque stability.

关 键 词：冠状动脉疾病 基质金属蛋白酶-1 基因多态性 

分 类 号：R654.14[医药卫生—外科学]