噪声性耳蜗损伤后豚鼠下丘γ-氨基丁酸(GABA)的改变  

Changes of gama-aminobutyric acid (GABA) in inferior colliculus in guinea pig following noise induced cochlear injury

作　　者：王丰[1] 刘良发[2] 洪波[3] 郭维[2] 刘会占[2] 韩东一[2] 

机构地区：[1]解放军总医院第一附属医院耳鼻咽喉科,北京100048 [2]解放军总医院耳鼻咽喉头颈外科,北京100853 [3]清华大学医学院生物工程系,北京100084

出　　处：《中华耳科学杂志》2010年第2期216-220,共5页Chinese Journal of Otology

基　　金：国家自然基金面上项目(编号:30872863);军队"十一.五"项目基金(编号:06H042)

摘　　要：目的研究噪声暴露耳蜗损伤后豚鼠下丘GABA(r-aminobutyric acid,r-氨基丁酸)神经元和GABA受体的改变。方法豚鼠被暴露于4kHz、1/3倍频程、100dB^120dB窄带噪声2~4小时建立噪声性耳蜗损伤的模型,用免疫组织化学ABC法(avidin-biotin complex,亲和素-生物素复合物法)检测声损伤后豚鼠下丘GABA神经元,计算IOD(integral optical density,积分光密度)值;用逆转录-多聚酶链反应(RT-PCR)方法检测GABAA/GABAB受体的含量。结果 (1)免疫组化染色结果显示,与阳性对照组(正常组)相比,噪声暴露后1天组GABA阳性神经元明显减少,但比阴性对照组明显增多。与之相应,噪声暴露后1天组的IOD值比阳性对照组(正常组)明显减少,比阴性对照组明显增多。(2)GABAA受体和GABAB受体的含量显示,噪声暴露后1天组和11天组明显减少,与正常组相比,差异有显著性(P<0.01),噪声暴露后21天组也明显减少,与正常组相比,差异有显著性(P<0.05)。结论噪声暴露后豚鼠下丘GABA神经递质的减少是电生理改变的物质基础之一。Objective To study changes of GABA-ergic neurons and GABA receptors in the inferior colliculus follo- wing noise exposure in guinea pigs. Methods 1） Cochlear injury was induced with 2-4 hour exposure to 1 / 3 octave band noise centered around 4 kHz at 100 - 120 dB SPL. 2） Avidin-biotin complex staining was used to determine the IOD value in GABA neurons. 3） RT-PCR was used to measure concentration of BABAA/ GABAB receptors. Results 1） Immunohistochemical staining indicated decreased number of GABA-positive neurons 1 day after noise exposure as compared to the positive （normal） control, although the number was still higher than that of the negative control. Similarly, IOD value at 1 day after noise exposure was also lower than the positive control and higher than the negative control. 2） BABAA/ GABAB receptors were also decreased on Day 1 and Day 11 post-exposure compared to the normal control （p 0.01）. They remained lower than the normal control on Day 21 post-exposure（p 0.05）. Conclusion Decrease of GABA neurotransmitter following noise exposure is one of the material basis for electrophysiological changes.

关 键 词：噪声 下丘 豚鼠 γ-氨基丁酸(GABA) 

分 类 号：R764.433[医药卫生—耳鼻咽喉科] R349.32[医药卫生—临床医学]