紫外线蓝光照射和树脂吸附清除血浆胆红素  

Clearance of plasma bilirubins isolated from artificial liver by ultraviolet-blue ray irradiation and resin adsorplion

作　　者：徐兵[1] 蒋晓织[1] 陈敏[1] 林曼芝[1] 林灼[1] 朱勇强[1] 

机构地区：[1]福州市传染病医院肝病研究所,350025

出　　处：《国际流行病学传染病学杂志》2010年第3期159-162,共4页International Journal of Epidemiology and Infectious Disease

摘　　要：目的探讨紫外线蓝光混合光照射血浆联合树脂吸附对血浆蛋白及胆红素的影响，初步建立紫外线蓝光和（或）树脂吸附净化血浆方法。方法将人工肝分离出的原血浆先用紫外线蓝光照射，再用D201型大孔强碱性阴离子交换树脂或D4006型非极性大孔吸附树脂吸附，根据采用的方法不同分为吸附组、照射组和照射吸附组，并与原血浆进行对照。观察在不同处理阶段（照射组、照射吸附组）及单纯吸附组蛋白和胆红素浓度变化。结果①照射组总胆红素（TBil）、直接胆红素（DBil）和间接胆红素（IBil）浓度明显低于原血浆组（P〈0．01），血浆蛋白照射前后差异无统计学意义（P〉0．05）。②用D201树脂吸附时，照射吸附组胆红素（TBil、DBil和IBil）清除率分别为85．3％、85．8％和85．1％，吸附组分别为58．3％、50．8％和61．7％，两组的胆红素清除率和浓度差异有统计学意义（P〈0．05，P〈0．01）；两组总蛋白、白蛋白差异无统计学意义（P〉0．05）。③用D4006树脂吸附时，照射吸附组胆红素（TBil、DBil和IBil）清除率分别为71．0％、74．5％和68．6％，吸附组分别为19．8％、17．6％和21．2％，两组胆红素清除率和浓度差异均有统计学意义（P〈0．01）；两组总蛋白、白蛋白差异无统计学意义（P〉0．05）。④在照射吸附组中，用D4006树脂时IBil清除率（68．6％）低于用D201树脂时IBil清除率（85．1％）（P〈0．05），使用D4006树脂组血浆自蛋白高于使用D201树脂组（P〈0．05）。结论紫外线蓝光照射血浆可直接降低胆红素浓度，明显提高阴离子交换树脂和吸附树脂胆红素清除率，对血浆蛋白回收影响较小，因此紫外线蓝光混合照射联合树脂吸附有望用于人工肝治疗。Objective To study the clearance of bilirubins from the plasma by ultraviolet-blue ray irradiation and resin adsorption. Methods The plasma from artifitial liver treatment were irradiated by the ultraviolet-blue ray and then filtrated the strongly basic anion exchange resin（D201） or adsorbent resin（D4006） to eliminate bilirubins. Results ① The mean level of total bilirubins（TBil）, direct bilirubin（DBil） and indirect bilirubin（IBil） decreased significantly after the irradiation（ P 〈 0.01 ）, and there was no significant decrease about albumin after the irradiation. ②For the group of irradiation then D201 adsorption, the clearance rates of TBil, DBil and IBil were 85.3%, 85.8% and 85.1% respectively; for the group of D201 adsorption without the irradiation, the rates were 58.3% ,50.8% and 61.7% respectively, and there was statistical difference between the two groups （ P 〈 0.05, P 〈 0.01）. There were no difference between two groups in the recovery rates of total protein and albumin（ P 〉 0.05）. ③For the group of irradiation then D4O01 adsorption, the clearance rates of TBil, DBil and IBil were 71.0%, 74.5% and 68.6% respectively; for the group of D4006 adsorption without the irradiation, the rates were 19.8%, 17.6% and 21.2% respectively, and there was statistical difference between the two groups （ P 〈 0.01 ）. There were no difference between two groups in the recovery rates of total protein and albumin（P 〉 0.05）. ④For the groups of irradiation followed by the resin adsorption, the clearance rate of IBil by D4006 （68.6%） was lower than that by D201 （85.1% ） （ P 〈 0.05）, but the recovery of albumin by D4006 was more than that by D201 （ P 〈 0.05）. Conclusions The ultraviolet-blue ray irradiation on plasma could directly decrease bilirubin concentrations and the irradiation followed by the resin adsorptions could decrease bilirubin concentrations further with a little influence to the recovery of albumin

关 键 词：紫外线 人工肝 阴离子交换树脂类 吸附 蛋白 胆红素 

分 类 号：R459.5[医药卫生—治疗学]