肾病Ⅰ号方对大鼠系膜细胞增殖及IL-6和FN表达的影响  

Effects of SBYHF on Proliferation and Expression of Fibronectin and Interleukin-6 in Rat Glomerular Mesangial Cells

作　　者：胡振奋[1] 程锦国[1] 董飞侠[1] 黄蔚霞[1] 冯如流[1] 陈春[1] 陈宇[1] 

机构地区：[1]浙江省温州市中医院肾内科,温州325000

出　　处：《中国中西医结合肾病杂志》2010年第1期21-24,共4页Chinese Journal of Integrated Traditional and Western Nephrology

基　　金：温州市科技局基金资助项目(No.Y20070050)

摘　　要：目的:观察肾病Ⅰ号方(SBYHF)对体外培养的大鼠肾小球系膜细胞(GMC)的增殖及表达纤维连接蛋白(FN)及白细胞介素-6(IL-6)的影响,探讨其抗肾纤维化的机制。方法:通过体外培养大鼠肾小球系膜细胞技术及体外药物血清实验方法,采用CCK-8法检测GMC的增殖,ELISA法检测FN的分泌水平,RT-PCR法检测IL-6 mRNA的表达。结果:肾病Ⅰ号方可显著抑制GMC和增殖(P<0.01),减少FN的分泌和IL-6 mRNA的表达(P<0.01)。结论:肾病Ⅰ号方可能通过减少GMC和FN的分泌,下调IL-6 mRNA表达,而抑制大鼠肾小球系膜细胞增殖,从而发挥抗肾脏纤维化的作用。Objective：To explore the molecular and cellular mechanism of SBYHF in ameliorating renal fibrosis through observing the effect of proliferation and expression of fibronectin and interleukin-6 in rat glomerular mesangial cells.Methods：The proliferation of rat GMC fostered in SBYHF was detected by CCK-8;the fibronectin（FN） secretion was detected by ELISA;and the Interleukin-6（IL-6） was detected by real time PCR.Results：SBYHF significantly inhibited GMC proliferation（P〈0.01） and decreased the expression of FN and IL-6（P〈0.01）.Conclusion：SBYHF may reduce the FN secretion and down-regulate the expression of IL-6 to inhibit the proliferation of rat glomerular mesangial Cells.This may be the mechanism of SBYHF in ameliorating renal fibrosis.

关 键 词：肾病Ⅰ号方 系膜细胞 白细胞介素-6 纤维连接蛋白 实时荧光定量PCR 

分 类 号：R285.5[医药卫生—中药学]