重组Galectin-9腺病毒对心脏移植小鼠Treg细胞分化和增殖的影响  

Influence of adenovirus carrying Galectin-9 gene on differentiation and proliferation of CD4^+CD25^+ regulatory T cells in mice with heart transplantation

作　　者：阳揭宇[1] 郭闻渊[1] 肖亮[1] 傅宏[1] 施晓敏[1] 刘芳[1] 丁国善[1] 傅志仁[1] 

机构地区：[1]第二军医大学长征医院器官移植科,上海200003

出　　处：《第二军医大学学报》2010年第6期595-598,共4页Academic Journal of Second Military Medical University

基　　金：国家自然科学基金面上项目(30772056)~~

摘　　要：目的观察重组Galectin-9腺病毒对小鼠心脏移植物生存期及调节性T(Treg)细胞分化和增殖的影响。方法建立小鼠心脏移植模型,随机均分为3组(n=10):对照组(术后用0.9%NaCl溶液)、空腺病毒组、重组Galectin-9腺病毒组,记录心脏移植物生存时间,于移植心停搏或术后14d取脾,分离单个核细胞,流式细胞仪检测CD4+CD25+Treg细胞比例。ELISA法检测外周血IL-10、TGF-β1浓度。结果与对照组、空腺病毒组相比,重组Galectin-9腺病毒组心脏移植物生存时间明显升高(P<0.01),重组Galectin-9腺病毒组CD4+CD25+Treg细胞比例明显升高(P<0.01)。重组Galectin-9腺病毒组IL-10、TGF-β1的含量明显高于对照组及空腺病毒组(P<0.01)。结论重组Galectin-9腺病毒能促进体内CD4+CD25+Treg细胞的分化与增殖,明显提高小鼠心脏移植物的生存时间。Objective To explore the influence of adenovirus carrying Galectin-9 gene on the survival of cardiac transplant and the differentiation and proliferation of CD4＋CD25＋Treg cells in mice receiving heterotopic heart transplantation.Methods Neck heterotopic heart transplantation models were established with male BALB/c→C57BL/6 mice by Cuff method,and they were randomly divided into 3 groups （n=10）：control group （received 0.9% sodium chloride solution after operation）,adenovirus group,and pAdeasyTM Galectin-9 treatment group.The survival time of the graft hearts was recorded.The spleens of mice were collected when the graft hearts stopped beating or 14 days after operation;the mononuclear cells were isolated and the proportion of CD4＋CD25＋ Treg cells was determined by flow cytometry.Treg cell related cytokines （IL-10,TGF-β1） in the peripheral blood were assayed by ELISA.Results The cardiac allografts in pAdeasyTM Galectin-9 group survived significantly longer than those in the other two groups （P0.01）,and the proportion of CD4＋CD25＋ Treg cells in CD4 ＋ T cells was significantly higher（P0.01）.The levels of IL-10 and TGF-β1 were increased in pAdeasyTM Galectin-9 group compared with control group and adenovirus group（P0.01）.Conclusion pAdeasyTM Galectin-9 can promote the proliferation and differentiation of CD4＋CD25＋ Treg cells in vivo,and can greatly improve the survival time of cardiac allograft after heart transplantation in mice.

关 键 词：GALECTIN-9 调节性T淋巴细胞 心脏移植 细胞分化 细胞增殖 

分 类 号：R392.4[医药卫生—免疫学]