HPLC法测定芪苓制剂超微粉中白术内酯Ⅲ的含量  

Quantitative determination of Actractylenolide Ⅲ in medicament of Qiling ultra micro powder by HPLC

作　　者：刘东艳[1] 李健[1] 马玉芳[1] 丘伟娟[1] 俞道进[1] 黄一帆[1] 

机构地区：[1]福建农林大学动物科学学院,福建福州350002

出　　处：《福建农林大学学报（自然科学版）》2010年第3期286-289,共4页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：福建省科技厅重大项目(2006NZ0003-1);福建省教育厅资助项目(JA08068)

摘　　要：芪苓制剂超微粉用甲醇提取,固相萃取小柱纯化,40%甲醇淋洗,60%甲醇洗脱后收集洗脱液.色谱条件:色谱柱ZORBAX SB-C18(4.6 mm×150 mm,5μm),流动相为甲醇-乙腈-水(30∶30∶40),流速1.0 mL.min-1,检测波长220 nm,柱温30℃.结果显示,白术内酯Ⅲ含量为0.77-27.02μg.mL-1时呈良好的线性关系(r2=0.9989),检测限为3.86 ng.mL-1,平均回收率为93.05%,RSD为4.04%.A high performance liquid chromatography（HPLC） method for determination of Actractylenolide Ⅲ in Qiling preparation ultra micro powder was established.Medicament of Qiling ultra micro powder was extracted with methanol,purified by solid-phase extraction,rinsed with 40%,eluted with 60% methanol,then concentrated.The optimal conditions for separation and detection were achieved on a C18 analytical column with an mobile phase consisted of methanol-acetonitrile-water（30∶30∶40） at the flow-rate of 1 mL·min-1.The detection wavelength was 220 nm.The column temperature was maintained at 30 ℃.The result indicated that the calibration curves for Actractylenolide Ⅲ were linear in the range of 0.77-27.02 μg·mL-1（r2=0.9989）,and the limit of quantification was 3.86 ng·mL-1.The recovery was 93.05% with RSD 4.04%.

关 键 词：芪苓制剂超微粉 高效液相色谱 白术内酯Ⅲ 固相萃取 

分 类 号：O657.72[理学—分析化学]