ELISA法与DELFIA法测定新生儿TSH的结果分析  被引量:1

Comparative analysis of TSH detection in neonates by ELISA and DELFIA

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作  者:郑红[1,2] 赵学勤[1,2] 杜红艳 张镜宇 王泽[1,2] 

机构地区:[1]天津医科大学内分泌研究所 [2]天津市儿童保健所

出  处:《中国地方病学杂志》1999年第1期67-69,共3页Chinese Jouranl of Endemiology

摘  要:目的比较双位点酶免法(Two-siteELISA)和时间分辨荧光免疫法(DELFIA)测定新生儿TSH的结果。方法采用芬兰进口试剂盒测定218例足月正常新生儿足血和脐血的成对TSH值。结果ELISA法和DELFIA法测定的脐血TSH值有明显的相关性,其相关系数r=0.91(t=32.41,P<0.001);两种方法测定的足血TSH值也有良好的相关性,其相关系数r=0.78(t=18.40,P<0.001);ELISA法测定值在足血和脐血间的相关系数却甚低,r=0.21(t=3.17,P<0.01);DELFIA法测定值在两者间的相关系数也很低,r=0.15(t=2.24,P<0.05)。结论ELISA和DELFIA两种方法测定的脐血和脐血之间、足血和足血之间均呈现良好的正相关。Objective To make comparation of cord and heel blood thyrod stimulating hormone levels in neonates.Methods A total pairs of TSH values in both cord and heel blood samples from 218 newborns were detemined by highly sensitive immunoassay (ELISA as well as DELFIA).Results The correlation coefficient of pair results by two methods are as follows:r=0.91(t=32.41,P<0.001);for cord bloods and r=0.78(t=18.40,P<0.001) for heel specimens,respectively.The correlation coefficient of cord and heel sample are as follows: r=0.21(t=3.17,P<0.01) for ELISA and r=0.15(t=2.24,P<0.05) for DELFIA,respectively.Conclusions It was demonstrated that the values detemined by two methods were significantly correlated,but those between cord and heel blood data were not so good.

关 键 词:THS ELISA DELFIA 新生儿 脐血 足血 

分 类 号:R446.112[医药卫生—诊断学] Q575.14[医药卫生—临床医学]

 

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